X-Message-Number: 25991
From: "Basie" <>
Subject: Venous systemic oxygen persufflation 
Date: Fri, 8 Apr 2005 23:58:31 -0400

I wonder if "Venous systemic oxygen persufflation" of the body will increase
better preservation.


Transplantation. 63(12):1843-1846, June 27, 1997.
Minor, Thomas 1,2; Klauke, Hiltrud 1; Vollmar, Brigitte 3; Isselhard, Wolf
1; Menger, Michael D. 3
Background. Venous systemic oxygen persufflation of the liver (i.e., gaseous
insufflation of oxygen via the venous vascular system) has proven to be an
effective tool for preventing anoxic tissue injury during extended time
periods of ischemic preservation. It also allows for an improved recovery of
the persufflated organ after orthotopic transplantation.

Methods. Biophysical aspects of the persufflation technique with regard to
persufflation pressure (9 mmHg versus 18 mmHg) and oxygen concentration
(pure oxygen versus air) in the persufflation gas were investigated in rat
livers, using epi-illumination microscopic detection of autofluorescence of
NADH, which accumulates in anoxic tissue.

Results. We demonstrated that a low-pressure persufflation (9 mmHg) is as
sufficient as a higher pressure persufflation (18 mmHg) in oxygenating the
ischemic organ. Moreover, oxygenation of the liver was found to be complete
and rather homogeneous upon the pure oxygen persufflation, irrespective of
the insufflation pressure used. In contrast, insufflation of air instead of
pure oxygen resulted in insufficient aeration of the liver, even at the
higher persufflation pressure of 18 mmHg.

Conclusions. Our results indicate that the oxygen concentration of the
persufflation gas rather than the persufflation pressure is a determinant of
successful tissue oxygenation during cold storage.

(C) Williams & Wilkins 1997. All Rights Reserved.

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