X-Message-Number: 26058 Date: Tue, 19 Apr 2005 18:41:20 -0700 (PDT) From: Doug Skrecky <> Subject: 172'nd update on fly longevity experiments This is the 172'nd update of my fly longevity experiments. Average temperature was 21.4 C during this run. Estimated maximal longevity using the formula (363 - T*11.2) is 123 days. Activated charcoal had been found to be slightly beneficial in previous runs. This run tests to see if it was the carbon fines (or lamp black) that constitute the active ingrediant. According to one survey, cranberry has the highest oxygen radical absorbance capacity (ORAC) among fruits and vegetables, while cloves have the highest ORAC of all food items. Although I'm not a believer in the "free radical theory of aging" mythos, I thought it might be interesting to give these two items a try. I'd tried some fruit juices which included a little cranberry, but had never tried the cranberry fruit itself. Unfortunately I'd forgotten that I'd already tried cloves, with no effect back in run #2. To my great surprise, the current batch of clove powder turned out to be highly toxic. I have no idea why this turned out to be the case. I suspect some minor contamination from the high dose clove bottle to the low dose cranberry bottle occurred, due to using the same tamping instrument on the fly food, without cleaning it after using it in the clove bottle. As expected the high dose cranberry proved to be largely neutral in its effects on longevity. The slight advantage in maximum longevity is likely a statistical artifact, which was derived from a single "lucky" fly. The poor results with lamp black rule out the idea that it was the fines from activated charcoal that were the source of its longevity benefit. Run #172 Percent Survival on Day supplement 12 26 33 45 55 64 71 78 83 87 91 95 101 ___________________________________________________________ control one 95 58 53 37 26 16 5 5 5 5 0 - - control two 88 56 44 36 36 16 8 0 - - - - - cloves 1/8 tsp 52 0 - - - - - - - - - - - " 1/4 tsp 29 0 - - - - - - - - - - - cranberrys 5% 76 33 29 14 0 - - - - - - - - " 20% 84 55 45 26 10 13 10 3 3 3 3 3 0 lamp black 1/8 tsp 83 39 39 11 11 0 - - - - - - - " 1/4 tsp 79 42 33 25 4 4 4 0 - - - - - " 1/2 tsp 86 59 36 14 5 5 5 0 - - - - - " 1 tsp 90 53 53 21 21 5 5 0 - - - - - Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=26058