X-Message-Number: 26070 Date: Fri, 22 Apr 2005 17:57:37 -0700 (PDT) From: Doug Skrecky <> Subject: 172'nd update on fly longevity experiments Message #26061 From: Kennita Watson <> Subject: Re: 172'nd update on fly longevity experiments > " If the food becomes too dry, the animals starve. They need to watch for 'cracked' and dry food and/or lifeless larvae. If this occurs, adding one or two drops of water will moisten the food and should solve the problem. On the other hand, the environment can become too humid and the food too moist. In this case the food will become contaminated with fungi, which is also detrimental to the flies. Finally, caution students not to leave the flies in direct sunlight or other extreme environmental conditions." < That's right, 4-24 fly food contains a mold inhibitor, but the risk of mold appears increases dramatically if the fly food has a wet surface. Dehydration exerts a powerful anorectic effect even in mammals, and I place additional plastic tape over the bottle lids to help prevent the fly food from drying out. Please note that the experimental area is kept in darkness to eliminate sunlight as a variable. >Confusion: I haven't seen anything online that discusses fruit fly lifespans longer than 80 days. Apparently it's normally about 35, and most articles talk about lengthening it with calorie restriction, lowered temperature, etc. to about 72. Where does a maximal longevity of 123 days come from, then? < The maximum longevity was derived from a simple equation which closely mirrors the effect of temperature on Oregon-R drosophila melanogaster's maximum longevity under good conditions. Flies can live as long as a year under low enough temperature conditions, so temperature adjustment is very important. Maximum longevity of 35 days has been obtained at high temperatures. My maximum experiment control longevities have usually been a little less than the calculated maximum longevity. Numerous interventions can increase longevity modestly, but no breakthrough (aside from that of temperature lowering) has been obtained yet. I'm hoping to change this state of affairs... Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=26070