X-Message-Number: 26082
Date: Sun, 24 Apr 2005 20:28:32 -0700 (PDT)
From: Doug Skrecky <>
Subject: 173'rd update on fly longevity experiments

     This is the 173'rd update of my fly longevity experiments. Average
 temperature was 21.6 C during this run. Estimated maximal longevity using
 the formula (363 - T*11.2) is 122 days.
     Hyaluronic acid has been dubbed a "rejuvenator". Here I give it the
 "fly test".
     The peroxynitrite scavenger urea slightly increased fly longevity in
 a published experiment. (Exp Gerontol 31(4): 533-544 1996) However unlike
 that experiment, the fly food I use is never changed after a run is
 started. Consequently, if urea is unstable in solution then it would be
 reasonable to expect some advantage only in the short term.
     In this run urea did indeed increase short term survival, but maximum
 longevity was reduced. I conclude that urea may be unstable in long
 term solution.
     Hyaluronic acid turned out to be a dud.

 Run #173                    Percent Survival on Day
 supplement              10 24 31 43 53 62 69 77 82 86 90
 ________________________________________________________
 control one             77 64 53 32 19 13  6  2  2  0  -
 control two             80 63 59 39 24 10  2  2  0  -  -
 hyaluronic acid 23 mg   71 59 56 29 24 12 10  2  0  -  -
   "             45 mg   91 66 63 40 20  9  3  3  3  3  0
   "             90 mg   77 55 42 26 16  3  3  0  -  -  -
   "            180 mg   82 70 70 33 27 18 15  9  3  0  -
 urea 1/8 tsp            89 81 69 50 33 14  3  0  -  -  -
   "  1/2 tsp            92 78 58 22  8  3  0  -  -  -  -
 yerbamate tea 2 bags/L  83 64 53 33  8  8  3  3  0  -  -
   "          10 bags/L  84 69 47 22 19  3  0  -  -  -  -

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