X-Message-Number: 26427 Date: Tue, 28 Jun 2005 19:23:37 -0700 (PDT) From: Doug Skrecky <> Subject: 181'st update on fly longevity experiments This is the 181'st update of my fly longevity experiments. Average temperature was 22.7 C during this run. Estimated maximal longevity using the formula (363 - T*11.2) is 109 days. Here a fermented soybean product called natto is tested. I also try adding baciilus natto directly to the fly food. Natto is regarded as a health food in Japan. It is a source of vitamin K2 or menaquinone, and also contains a fibrolytic enzyme called nattokinase. Bacillus natto also contains K2, but has little nattokinase. High doses of natto mixed with fly food reduced the ability of the food to hold water, so that the surface became somewhat wet. Possibly for this reason, natto turned out to be mildly toxic. On the other hand bacillus natto had no effect on food hydration, and a high dose even gave the appearance of being beneficial. Bacillus natto is being retested in run #188. Run #181 Percent Survival on Day supplement 11 19 28 36 46 54 60 67 75 83 93 _________________________________________________________ control one 96 80 61 47 37 24 20 12 4 0 - control two 100 85 60 60 48 27 15 8 2 0 - natto 2.5% 94 74 62 48 28 14 8 2 2 0 - " 5% 100 85 80 59 44 31 8 0 - - - " 10% 91 94 88 72 44 31 3 3 0 - - " 20% 96 91 85 67 20 15 7 0 - - - bacillus natto 1/16 tsp 98 81 67 65 49 30 14 7 5 0 - " 1/8 tsp 98 88 74 52 48 26 10 5 5 0 - " 1/4 tsp 100 93 64 53 42 33 22 11 4 2 0 " 1/2 tsp 100 97 76 74 74 74 66 42 24 5 0 Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=26427