X-Message-Number: 26654 Date: Sun, 17 Jul 2005 14:57:17 -0700 (PDT) From: "D. den Otter" <> Subject: Re: chemopreservation (reply to Doug Skrecky) Doug Skrecky wrote, regarding plastination & defatting: <<Prior to plastic infiltration, aldehyde fixed tissue must be completely dehydrated in alcohol. Both alcohol as well as the plastic monomer destroy cell membranes. From the viewpoint of optimal preservation this is far inferior to anhydroprotectant infiltration and osmotic dehydration, followed by vaccuum dessication. Some options such as plastination can be eliminated from consideration immediately, due to their great inferiority to alternative inexpensive preservation techniques.>> Could you perhaps describe this anhydroprotectant infiltration - osmotic dehydration - vacuum dessication procedure in greater detail? So basically substances and equipment used, approx. pricing (where appropriate), how long each step would take, recommended storage temperature etc. Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=26654