X-Message-Number: 26654
Date: Sun, 17 Jul 2005 14:57:17 -0700 (PDT)
From: "D. den Otter" <>
Subject: Re: chemopreservation (reply to Doug Skrecky)

Doug Skrecky wrote, regarding plastination &
defatting:

<<Prior to plastic infiltration, aldehyde fixed tissue
must be completely dehydrated in alcohol. Both alcohol
as well as the plastic monomer destroy cell membranes.
From the viewpoint of optimal preservation this is far
inferior to anhydroprotectant infiltration and osmotic
dehydration, followed by vaccuum dessication. Some
options such as plastination can be eliminated from
consideration immediately, due to their great
inferiority
to alternative inexpensive preservation techniques.>>

Could you perhaps describe this anhydroprotectant
infiltration - osmotic dehydration - vacuum
dessication procedure in greater detail? So basically
substances and equipment used, approx. pricing (where
appropriate), how long each step would take,
recommended storage temperature etc.

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