X-Message-Number: 26664 Date: Tue, 19 Jul 2005 19:05:36 -0700 (PDT) From: Doug Skrecky <> Subject: optimal intake of folic acid is unknown [Folic acid sounds great. It lowers blood pressure, cholesterol, as well as IMT, which is a measure of atherosclerosis. The kicker is that is may also increase the risk of dementia.] Atherosclerosis. 2005 Jul;181(1):131-5. Epub 2005 Feb 16. Decrease of carotid intima-media thickness in patients at risk to cerebral ischemia after supplementation with folic acid, Vitamins B6 and B12. OBJECTIVE:: Hyperhomocysteinemia is associated with atherosclerotic risk. Although vitamins can lower homocysteine (Hcy), information about effects on atherosclerosis is scarce. METHODS:: We used carotid intima-media thickness (IMT) as an accepted marker of atherosclerotic changes. Fifty patients (60+/-8 years) with IMT>/=1mm were included. In a double blind, randomized trial they received daily 2.5mg folic acid, 25mg Vitamin B6, and 0.5mg Vitamin B12 or placebo for 1 year. RESULTS:: In the treatment group, Hcy decreased from 10.50+/-3.93 to 6.56+/-1.53mumol/l (P<0.0001), whereas it remained unchanged in the placebo group (10.76+/-2.36 versus 10.45+/-3.30mumol/l). IMT decreased from 1.50+/-0.44 to 1.42+/-0.48mm (P=0.034) in the treatment group, whereas it increased from 1.47+/-0.57 to 1.54+/-0.71mm in the placebo group. The mean individual changes of IMT between both groups differed significantly (-0.08+/-0.17 versus 0.07+/-0.25mm, P=0.019). Multiple regression analysis revealed that the observed effect on IMT depended only on medication. CONCLUSIONS:: Vitamin supplementation significantly reduces IMT in patients at risk. This effect is independent of Hcy concentration. J Clin Endocrinol Metab. 2005 May 17; [Epub ahead of print] L-FOLIC ACID SUPPLEMENTATION IN HEALTHY POSTMENOPAUSAL WOMEN: EFFECT ON HOMOCYSTEINE AND GLYCO-LIPID METABOLISM. Context. Hyperhomocisteinemia as well as alterations of the glycemic and lipidic metabolism are recognized as risk factors for cardiovascular diseases. Objective. To study the effect of L-folic acid supplementation on homocysteine (Hcy) and related thiols such as cysteine (Cys) and cysteinil-glicine (Cys-Glyc) pathways and their relationship with glucose, insulin and lipidic metabolism in normoinsulinemic postmenopausal women. Design. Randomized placebo not double blind trial. Setting. Accademic research center. Patients or Other Partecopants. Twenty healthy postmenopausal women were selected. No patient was taking drugs known to affect the lipid or glucose metabolism. Intervention (s). Patients underwent two hospitalizations before and after 8 weeks of L-acid folic (7.5 mg/d) or placebo administration. The glycemic metabolism was studied by an oral glucose tolerance test (OGTT) and a hyperinsulinemic euglycemic clamp. The homocysteine metabolism was studied by a standardized oral methionine loading test. Main Outcome Measure (s). Homocysteine, cysteine (Cys) and cysteinil-glicine (Cys-Glyc) basal and post metionine loading test were measured. Basal insulin, glucose, Pep-C levels as well as AUC-I, AUC -pep, FHIE, M were assayed. The total Col, HDL LDL levels and the Cholesterol/HDL and LDL/HDL ratio were also measured Results. Total basal homocysteine (tHcy) concentration and plasma post-methionine loading Hcy values significantly decreased (P < 0.01) in L-folic acid treated patients whereas post methionine loading Cys-Glyc levels markedly increased (P < 0.02). Furthermore L-folic acid intake induced a significant improvement of the carbohydrate metabolism through an increase of fractional hepatic insulin extraction (P < 0.05) and peripheral insulin sensitivity (P < 0.02) in normoinsulinemic women. HDL levels considerably increased inducing an improvement of other atherosclerotic indexes such as Cholesterol/HDL and LDL/HDL ratio (P < 0.03). Conclusions. These results show that folic acid supplementation lowers plasma Hcy levels and improves insulin and lipid metabolism reducing the risk of cardiovascular disease. J Am Coll Cardiol. 2005 May 17;45(10):1580-4. Epub 2005 Apr 26 High-dose folic acid acutely improves coronary vasodilator function in patients with coronary artery disease. OBJECTIVES: We investigated the acute effect of orally administered high-dose folic acid on coronary dilator function in humans. BACKGROUND: Folic acid and its active metabolite, 5-methyltetrahydrofolate, increase endothelium-dependent vasodilation in human peripheral circulation. However, the acute effect on coronary circulation is not known. METHODS: Fourteen patients with ischemic heart disease, age 62 +/- 12 years (mean +/- SD), were enrolled in a double-blind, placebo-controlled crossover trial. Basal and adenosine-stimulated myocardial blood flow (MBF) were determined by positron emission tomography, and myocardial flow reserve was calculated. Each patient was studied after ingestion of placebo and after ingestion of 30 mg folic acid. Myocardial zones were prospectively defined physiologically as "normal" versus "abnormal" on the basis of MBF response to adenosine 140 microg/kg/min (normal = MBF >1.65 ml/min/g). Abnormal and normal zones were analyzed separately in a patient-based analysis. RESULTS: Folate was associated with a reduction in mean arterial pressure (100 +/- 12 mm Hg vs. 96 +/- 11 mm Hg, placebo vs. folate, p < 0.03). Despite the fall in mean arterial pressure, folic acid significantly increased the MBF dose response to adenosine (p < 0.001 using analysis of variance) in abnormal zones, whereas MBF in normal zones did not change. In abnormal segments, folic acid increased peak MBF by 49% (1.45 +/- 0.59 ml/min/g vs. 2.16 +/- 1.01 ml/min/g, p < 0.02). Furthermore, folate increased dilator reserve by 83% in abnormal segments (0.77 +/- 0.59 vs. ml/min/g 1.41 +/- 1.08 ml/min/g, placebo vs. folate, p < 0.05), whereas dilator reserve in normal segments remained unchanged (2.00 +/- 0.61 ml/min/g vs. 2.12 +/- 0.69 ml/min/g, placebo vs. folate, p = NS). CONCLUSIONS: The data demonstrate that high-dose oral folate acutely lowers blood pressure and enhances coronary dilation in patients with coronary artery disease. Arch Neurol. 2005 Apr;62(4):641-5. Dietary folate and vitamin B12 intake and cognitive decline among community-dwelling older persons. BACKGROUND: Deficiencies in folate and vitamin B12 have been associated with neurodegenerative disease. OBJECTIVE: To examine the association between rates of age-related cognitive change and dietary intakes of folate and vitamin B12. DESIGN: Prospective study performed from 1993 to 2002. SETTING: Geographically defined biracial community in Chicago, Ill. PARTICIPANTS: A total of 3718 residents, 65 years and older, who completed 2 to 3 cognitive assessments and a food frequency questionnaire. MAIN OUTCOME MEASURE: Change in cognitive function measured at baseline and 3-year and 6-year follow-ups, using the average z score of 4 tests: the East Boston Tests of immediate and delayed recall, the Mini-Mental State Examination, and the Symbol Digit Modalities Test. RESULTS: High folate intake was associated with a faster rate of cognitive decline in mixed models adjusted for multiple risk factors. The rate of cognitive decline among persons in the top fifth of total folate intake (median, 742 microg/d) was more than twice that of those in the lowest fifth of intake (median, 186 microg/d), a statistically significant difference of 0.02 standardized unit per year (P = .002). A faster rate of cognitive decline was also associated with high folate intake from food (P for trend = .04) and with folate vitamin supplementation of more than 400 microg/d compared with nonusers (beta = -.03, P<.001). High total B12 intake was associated with slower cognitive decline only among the oldest participants. CONCLUSIONS: High intake of folate may be associated with cognitive decline in older persons. These unexpected findings call for further study of the cognitive implications of high levels of dietary folate in older populations. 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