X-Message-Number: 26733 Date: Fri, 29 Jul 2005 18:09:11 -0700 (PDT) From: Doug Skrecky <> Subject: 184'th update on fly longevity experiments This is the 184'th update of my fly longevity experiments. Average temperature was 22.9 C during this run. Estimated maximal longevity (EML) using the formula (363 - T*11.2) is 107 days. This run checks out beta-hydroxy beta-methyl butyrate (HMB), and metformin. HMB is a muscle building agent, which may have some utility in the treatment of human sarcopenia. In contrast to mammals with increased age, flies suffer from motor neuron degeneration, rather than muscle atropy. Metformin is a prescription anti-diabetic drug, with some homologies with caloric restriction in mammals. In contrast caloric restriction appears to offer no significant benefit to flies. Thanks to B.R. for the gift of metformin. Neither HMB nor metformin offered any benefit. However at just 63 days maximum survival does not compare well with the EML of 107 days. In the past I have noticed that my own experiments tend to closely approach EML during the winter months, but tend to be more variable during the summer when a significant deficit with respect to EML is common. The causal factors behind this are under investigation. Run #182 Percent Survival on Day supplement 8 16 26 34 40 47 55 63 73 ______________________________________________ control one 96 92 77 65 50 35 15 8 0 control two 97 91 68 62 47 27 6 0 - HMB 47 mg 100 87 57 57 44 26 13 0 - " 188 mg 94 88 63 63 38 13 0 - - " 750 mg 96 69 54 46 35 15 8 4 0 metformin 1/32 gm 92 76 60 56 28 12 0 - - " 1/16 gm 100 89 63 63 47 26 11 5 0 " 1/8 gm 100 75 63 54 38 8 0 - - " 1/4 gm 90 74 47 42 32 21 0 - - " 1/2 gm 88 53 47 47 41 18 12 12 0 Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=26733