X-Message-Number: 26951
Date: Wed, 7 Sep 2005 11:49:55 +0200
From: Eugen Leitl <>
Subject: Re: [CN] chemical fixation
References: <>

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On Tue, Sep 06, 2005 at 07:19:08PM -0700, Jordan Sparks wrote:

> One thing I forgot to mention.  Oregon Cryo will probably use a
> combination of chemical fixation (gluteraldehyde) and vitrification for

How do you propose preparing fresh glutaraldehyde in the field?
Alternatively, who's maintaining fresh stock solution?
Will glutaraldehyde still crosslink well with a high molality of polyols
and other cryoprotectants present? Why glutaraldehyde, and not
some other fixation protocol? What is the precise protocol, and
what is the justification for using it? Will fixation result in 

artifacts and complications? Will simultaneous fixation greatly reduce the final
concentration of cryoprotectant in the tissue, especially the
brain tissue? How well does fixation play with vitrification?

> our biostasis protocol.  I believe in over engineering everything.  We
> are interested in preserving structure, and chemical fixation is very

How do you know it is very good at that?

> good at that.  Current vitrification techniques will require
> nanotechnology anyway, so a few cross links shouldn't slow them down
> any.

-- 
Eugen* Leitl <a href="http://leitl.org">leitl</a>
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ICBM: 48.07100, 11.36820            http://www.leitl.org
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