X-Message-Number: 26951 Date: Wed, 7 Sep 2005 11:49:55 +0200 From: Eugen Leitl <> Subject: Re: [CN] chemical fixation References: <> --Gg+kmP6Jtal7S17t Content-Disposition: inline On Tue, Sep 06, 2005 at 07:19:08PM -0700, Jordan Sparks wrote: > One thing I forgot to mention. Oregon Cryo will probably use a > combination of chemical fixation (gluteraldehyde) and vitrification for How do you propose preparing fresh glutaraldehyde in the field? Alternatively, who's maintaining fresh stock solution? Will glutaraldehyde still crosslink well with a high molality of polyols and other cryoprotectants present? Why glutaraldehyde, and not some other fixation protocol? What is the precise protocol, and what is the justification for using it? Will fixation result in artifacts and complications? Will simultaneous fixation greatly reduce the final concentration of cryoprotectant in the tissue, especially the brain tissue? How well does fixation play with vitrification? > our biostasis protocol. I believe in over engineering everything. We > are interested in preserving structure, and chemical fixation is very How do you know it is very good at that? > good at that. Current vitrification techniques will require > nanotechnology anyway, so a few cross links shouldn't slow them down > any. -- Eugen* Leitl <a href="http://leitl.org">leitl</a> ______________________________________________________________ ICBM: 48.07100, 11.36820 http://www.leitl.org 8B29F6BE: 099D 78BA 2FD3 B014 B08A 7779 75B0 2443 8B29 F6BE --Gg+kmP6Jtal7S17t Content-Description: Digital signature Content-Disposition: inline -----BEGIN PGP SIGNATURE----- Version: GnuPG v1.4.1 (GNU/Linux) iD8DBQFDHrfDdbAkQ4sp9r4RAkvEAJsHREDQP5eGDTii0wuBZhE7fPlgVwCfR2o3 k48TdU8EwDpaN09i/hf8KWY=qtAo -----END PGP SIGNATURE----- --Gg+kmP6Jtal7S17t-- Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=26951