X-Message-Number: 26982
From: David Stodolsky <>
Subject: Cryopreservation of ovarian tissue
Date: Fri, 9 Sep 2005 18:57:15 +0200

Cryopreservation of ovarian tissue from girls and women prior to  
treatment of a malignant disease
Kirsten L. Tryde Schmidt's Ph.D. dissertation

One finding was that follicles survived transport for four hours  
cooled with water ice.

Summary in Danish:

http://www.sund.ku.dk/ph.d.-forsvar/resumeKirstenTrydeSchmidt.htm


Results appeared in five articles, some in English. One is:

<http://www.ingentaconnect.com/content/repro/rebi/ 
2004/00000008/00000004/art00012;jsessionid=22efdd21nrvug.victoria>

Kirsten L. Tryde Schmidt1; Claus Yding Andersen; J rgen Starup; Anne  
Loft; Anne Grete Byskov; Anders Nyboe Andersen

Reproductive BioMedicine Online, Volume 8, Number 4, 1 April 2004,  
pp. 448-453(6).

Orthotopic autotransplantation of cryopreserved ovarian tissue to a  
woman cured of cancer   follicular growth, steroid production and  
oocyte retrieval

Abstract:
Cryopreservation of human ovarian tissue is now an option for cancer  
patients facing treatment with gonadotoxic regimes, as a means of  
preserving their fertility. So far, there have been only a few  
reports on autotransplantation of frozen thawed tissue with regard to  
restoration of ovarian function. The present report describes a 32- 
year-old woman diagnosed with Hodgkin's lymphoma, who had  
cryopreserved ovarian tissue transplanted orthotopically after  
secondary ovarian failure due to chemotherapy. Only 8 weeks after  
transplantation, ultrasonography of the remaining ovary revealed two  
follicles with diameters of 10 and 15 mm. Concomitantly, circulating  
concentrations of oestradiol increased, while concentrations of  
gonadotrophins decreased. In the following months, the patient  
menstruated three times. Subsequent pituitary down-regulation with a  
gonadotrophin-releasing hormone (GnRH) agonist and ovarian  
stimulation resulted in development of one pre-ovulatory follicle  
from which a metaphase II oocyte was retrieved; however, this oocyte  
was unable to sustain further development after intracytoplasmic  
sperm injection (ICSI). Intrafollicular concentrations of oestradiol  
and progesterone suggested a normal luteinizing response of the  
follicle to human chorionic gonadotrophin stimulation. A 7-month  
follow-up revealed continued vivid follicular activity and normal  
oestradiol concentrations. In conclusion, cryopreserved human ovarian  
tissue restored ovarian function for several cycles and sustained  
development of mature oocytes in a woman cured of cancer.




dss

David Stodolsky    Skype: davidstodolsky

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