X-Message-Number: 27646 Date: Tue, 21 Feb 2006 20:34:00 -0800 (PST) From: Doug Skrecky <> Subject: lithium antagonizes one aspect of aging [Upregulating autophagy may prevent some age-associated problems, such as cardiac enlargement. Increased autophagy can be achieved the hard way, by means of either caloric restriction, or alternatively by periodic fasting, without any net reduction in total caloric intake. Lithium offers a much easier alternative means for achieving increased autophagy. Autophagy is greatly reduced in old age.] J Cell Biol. 2005 Sep 26;170(7):1101-11. Lithium induces autophagy by inhibiting inositol monophosphatase. Macroautophagy is a key pathway for the clearance of aggregate-prone cytosolic proteins. Currently, the only suitable pharmacologic strategy for up-regulating autophagy in mammalian cells is to use rapamycin, which inhibits the mammalian target of rapamycin (mTOR), a negative regulator of autophagy. Here we describe a novel mTOR-independent pathway that regulates autophagy. We show that lithium induces autophagy, and thereby, enhances the clearance of autophagy substrates, like mutant huntingtin and alpha-synucleins. This effect is not mediated by glycogen synthase kinase 3beta inhibition. The autophagy-enhancing properties of lithium were mediated by inhibition of inositol monophosphatase and led to free inositol depletion. This, in turn, decreased myo-inositol-1,4,5-triphosphate (IP3) levels. Our data suggest that the autophagy effect is mediated at the level of (or downstream of) lowered IP3, because it was abrogated by pharmacologic treatments that increased IP3. This novel pharmacologic strategy for autophagy induction is independent of mTOR, and may help treatment of neurodegenerative diseases, like Huntington's disease, where the toxic protein is an autophagy substrate. Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=27646