X-Message-Number: 27727
Date: Fri, 17 Mar 2006 19:40:25 -0800 (PST)
From: Doug Skrecky <>
Subject: inhibition of brain edema by quercetin liposomes

[Quercetin by itself does not cross the blood-brain barrier. Like
resveratrol, quercetin is effective in vitro for increasing
(antiaging) sirtuin levels. However unlike (expensive) resveratrol,
quercetin does not increase animal lifespan. There is a possibility
that liposome encapsulated quercetin might perform differently in this
regard, since many aging processes are thought to be mediated through
the central nervous system.]

Mech Ageing Dev. 2006 Apr;127(4):391-7. Epub 2006 Feb 9.
Mannosylated liposomal flavonoid in combating age-related
ischemia-reperfusion induced oxidative damage in rat brain.
  Active oxygen species alter the activities of the enzymes involved in
the defence against free radicals and substantially influence the aging
process and age-dependant neuropathology. Unilamellar liposomes were used
to deliver flavonoidal antioxidant quercetin (QC) to rat
brain. Antioxidant potential of QC loaded in mannosylated (QC
7.2mumol/kgb.wt.) liposomes (50nm) was investigated by an in vivo model
of cerebral ischemia and reperfusion on Sprague Dawley young (2 months
old, b.wt. 160-180g) and aged (20 months old,
b.wt. 415-440g) rats. Animals were made ischemic for 30min by
bilateral clamping of the common carotid artery followed by a 30min
cerebral reperfusion by withdrawing the clamping. Diene level and
(GSSG/GSH) ratio were found to be higher in normal aged, compared to
normal young rat brain. Superoxide dismutase, catalase,
glucose-6-phosphate dehydrogenase, glutathione reductase and glutathione
S-transferase activities were lower in normal aged rat brain. Further
reduction of these antioxidant enzymes was observed in aged rat brain by
the induction of cerebral ischemia and reperfusion. Mannosylated
liposomally encapsulated QC treatment resulted in a significant
preservation of the activities of antioxidant enzymes and a marked
inhibition of cellular edema formation in neuronal cells of young and old

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