X-Message-Number: 27755
Date: Fri, 24 Mar 2006 19:25:21 -0800 (PST)
From: Doug Skrecky <>
Subject: improving cold preservation with flavonoids

Transplantation. 2006 Jan 27;81(2):231-9.
Improved cold preservation of kidney tubular cells by means of adding
bioflavonoids to organ preservation solutions.
  BACKGROUND: Cold ischemia and reperfusion during renal transplantation
result in release of reactive oxygen species. The aim of this study is to
examine whether cold storage induced cell injury can be ameliorated by
adding flavonoids directly to preservation solutions. METHODS: Cultured
renal tubular epithelial cells (LLC-PK1) were stored in University of
Wisconsin (UW) or Euro-Collins (EC) solution at 4 degrees C for 20
hours. Preservation solutions were supplemented with various
flavonoids. After rewarming, structural and metabolic cell integrity was
measured by lactate dehydrogenase (LDH) release and MTT-test, and lipid
peroxidation was assessed from generation of thiobarbituric acid-reactive
substances (TBARS). RESULTS: Twenty hours of cold storage resulted in a
substantial loss of cell viability in both preservation solutions (in
EC: LDH release 92.4+/-2.7%; MTT-test 0.5+/-0.7%). Addition of luteolin,
quercetin, kempferol, fisetin, myricetin, morin, catechin, and silibinin
significantly reduced cell injury (for luteolin in EC: LDH release
2.4+/-1.6%; MTT-test 110.3+/-10.4%, P<0.01; TBARS-production (related to
cold stored control cells) 8.9+/-2.6%). No cytoprotection was found for
apigenin, naringenin, and rutin. Protective potency of flavonoids depends
on number of hydroxyl-substituents and lipophilicity of the diphenylpyran
compounds. CONCLUSION: Cold storage induced injury of renal tubular cells
was substantially ameliorated by adding selected flavonoids directly to
preservation solutions.

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