X-Message-Number: 27883 Date: Sun, 30 Apr 2006 09:46:01 -0700 (PDT) From: Doug Skrecky <> Subject: CR and brain aging [Considering the global effects of CR, one might be excused from assuming that this intervention would have far more dramatic effects on animal longevity than it does. A number of studies in adult animals have found no longevity advantage to CR at all. One possible explanation for these unexpected findings may be due to CR not affect the aging of the central nervous system. Since the energy supply of this tissue is preferentially preserved, caloric consumption by the brain can not be restricted due to simple dietary alterations. However if the functioning of the central nervous system is preserved by whatever means, then this might have a far more powerful effect on longevity through prolonged maintainance of the stem cell niche, via continued exposure to a youthful (brain-derived) hormonal environment. This might explain of the potent antiaging effect of chromium picolinate. The following abstract illustrates the lack of effect of CR on brain aging.] Ann N Y Acad Sci. 2004 Jun;1019:269-73. Measurement of the 4,834-bp mitochondrial DNA deletion level in aging rat liver and brain subjected or not to caloric restriction diet. Several studies have demonstrated an age-related accumulation of the amount of a specific 4834-bp mitochondrial DNA (mtDNA) deletion in different tissues of rat (liver, brain, and skeletal muscle). We investigated the influence of a caloric restriction diet (CR) on a selected age-associated marker of mtDNA damage, as the 4834-bp deletion, using quantitative real-time PCR. The mtDNA deleted level has been determined with respect to the mitochondrial D-loop level, using specific primers and TaqMan probes for each target. In liver we found an age-related increase of the deletion level (twofold) that was reversed and brought back to the adult level by a CR diet. On the contrary, in the brain the age-related increase of the deletion level (eightfold) was not affected by CR at all. The different effect of the CR on the deletion level in liver and brain might be a further element supporting the tissue-specificity of the aging process. Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=27883