X-Message-Number: 27993
Date: Thu, 25 May 2006 12:56:44 -0700 (PDT)
From: Doug Skrecky <>
Subject: Lactamide as a novel cryoprotectant

[Reality check: lactamide is very expensive$$ I haven't read the full
report yet, but I find it interesting that the results for DMSO are not
mentioned in the abstract.]

J Reprod Dev. 2006 May 8; [Epub ahead of print]
Comparison of Glycerol, Lactamide, Acetamide and
Dimethylsulfoxide as Cryoprotectants of Japanese White Rabbit
  The rabbit is considered to be a valuable laboratory animal. We
compared glycerol, lactamide, acetamide, and dimethylsulfoxide
(DMSO) as cryoprotectants in egg-yolk diluent of ejaculated
Japanese white rabbit spermatozoa for improvement of sperm
cryopreservation methods. Rabbit semen was frozen with 1.0 M
glycerol, lactamide, acetamide, or DMSO in plastic straws.
Forward progressive motility and plasma membrane integrity of the
post-thaw spermatozoa were examined. The rate of forward
progressive motile spermatozoa in lactamide (37.8 +/- 3.0%) was
significantly (P<0.05) higher than in glycerol (17.0 +/- 3.3%).
In addition, the rates of sperm plasma membrane integrity in
lactamide and acetamide (35.9 +/- 3.3% and 30.2 +/- 3.0%,
respectively) were significantly (P<0.05) higher than in glycerol
(17.0 +/- 2.6%). The results indicate that 1.0 M lactamide and
acetamide have higher cryoprotective effects than 1.0 M glycerol
for cryopreservation of Japanese white rabbit spermatozoa.

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