X-Message-Number: 28336 Date: Sun, 20 Aug 2006 17:25:57 -0700 (PDT) From: Doug Skrecky <> Subject: 229'th update on fly longevity experiments This is the 229'th update of my fly longevity experiments. Average temperature was 24.0 C during this run. Estimated maximal longevity using the formula (363 - T*11.2) is 94 days. High intensity sweeteners were a surprise hit in run #217. At the highest dosage all sweeteners were effective in increasing maximum lifespan. This time yet higher doses were tested, with the previous high dosage, now being the lowest tested dosage. Unfortunately control longevity was very poor in this run, which implicates pathogen contamination in the breeding bottle used. However once again 4 tabs of either aspartame or saccharin, and the 1 tsp dosage of cyclamate increased maximum lifespan. Although higher dosages of proved to be slightly toxic, it is nonetheless remarkable that at a certain dosage all three of these sweeteners were able to exert a consistent benefit. The only question that needs to be answered now is why they work. Run #229 Percent Survival on Day supplement 8 19 31 39 47 60 ___________________________________ control one 78 50 17 0 - - control two 80 41 14 2 0 - aspartame 4 tabs 97 56 19 4 3 0 " 8 tabs 88 43 18 7 4 0 " 16 tabs 78 36 21 8 2 0 cyclamate 1 tsp 78 39 20 6 3 0 " 2 tsp 70 25 12 5 3 0 " 4 tsp 62 7 7 0 - - saccharin 4 tabs 80 44 20 3 3 0 " 8 tabs 84 24 11 4 2 0 " 16 tabs 67 46 19 8 0 - Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=28336