X-Message-Number: 29313
Date: Thu, 15 Mar 2007 10:37:03 -0800 (PST)
From: 
Subject: inhibitors of warm ischemia damage

Nephron Physiol. 2007 Mar 1;105(4):p57-p65 [Epub ahead of print]
Role of Combined L-Arginine and Prostaglandin E(1) in Renal
Ischemia-Reperfusion Injury.
Mahmoud IM, Hussein AE, Sarhan ME, Awad AA, El Desoky I. Urology and
Nephrology Center, Mansoura University, Mansoura, Egypt.
    Background:L-Arginine (L-arg) and Prostaglandin E(1) (PGE(1)) have
been used effectively as single agents to ameliorate renal
ischemia-reperfusion injury. We hypothesized that combined treatment with
L-arg and PGE(1 )would be more effective. Materials and Methods: The left
renal artery of male Sprague-Dawley rats was clamped for 45 min and the
right kidney was removed. Fifty six rats were randomly allocated into 5
groups each consisted of 12 rats except sham group (n = 8). (1) sham,
underwent right nephrectomy only; (2) control, untreated ischemic
rats; (3) L-arg group, L-arg-treated ischemic rats; (4) PGE(1) group,
PGE(1)-treated ischemic rats; (5) L-arg+PGE(1) group, ischemic rats
treated with both L-arg and PGE(1). Renal function and histology were
assessed on days 2 and 7 postoperatively. Results: All rats, except
control ones, showed a significant improvement of renal function towards
normal on postoperative day 7. Serum creatinine and creatinine clearance
were significantly better in L-arg+PGE(1) group compared to all other
groups on day 7. With the exception of sham-operated and
L-arg+PGE(1)-treated animals, all other groups showed significant
increases in fractional excretion of sodium (FE(Na)) in response to renal
ischemia-reperfusion. The severest tubular damage was determined in the
kidneys of control rats. Rats treated with L-arg+PGE(1) had the least
severe tubular damage. Conclusion: The administration of either L-arg or
PGE(1) attenuates both functional and structural consequences of renal
warm ischemia. A near total protection might be achieved when both agents
are administered concomitantly.
PMID: 17337910

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