X-Message-Number: 29313 Date: Thu, 15 Mar 2007 10:37:03 -0800 (PST) From: Subject: inhibitors of warm ischemia damage Nephron Physiol. 2007 Mar 1;105(4):p57-p65 [Epub ahead of print] Role of Combined L-Arginine and Prostaglandin E(1) in Renal Ischemia-Reperfusion Injury. Mahmoud IM, Hussein AE, Sarhan ME, Awad AA, El Desoky I. Urology and Nephrology Center, Mansoura University, Mansoura, Egypt. Background:L-Arginine (L-arg) and Prostaglandin E(1) (PGE(1)) have been used effectively as single agents to ameliorate renal ischemia-reperfusion injury. We hypothesized that combined treatment with L-arg and PGE(1 )would be more effective. Materials and Methods: The left renal artery of male Sprague-Dawley rats was clamped for 45 min and the right kidney was removed. Fifty six rats were randomly allocated into 5 groups each consisted of 12 rats except sham group (n = 8). (1) sham, underwent right nephrectomy only; (2) control, untreated ischemic rats; (3) L-arg group, L-arg-treated ischemic rats; (4) PGE(1) group, PGE(1)-treated ischemic rats; (5) L-arg+PGE(1) group, ischemic rats treated with both L-arg and PGE(1). Renal function and histology were assessed on days 2 and 7 postoperatively. Results: All rats, except control ones, showed a significant improvement of renal function towards normal on postoperative day 7. Serum creatinine and creatinine clearance were significantly better in L-arg+PGE(1) group compared to all other groups on day 7. With the exception of sham-operated and L-arg+PGE(1)-treated animals, all other groups showed significant increases in fractional excretion of sodium (FE(Na)) in response to renal ischemia-reperfusion. The severest tubular damage was determined in the kidneys of control rats. Rats treated with L-arg+PGE(1) had the least severe tubular damage. Conclusion: The administration of either L-arg or PGE(1) attenuates both functional and structural consequences of renal warm ischemia. A near total protection might be achieved when both agents are administered concomitantly. PMID: 17337910 Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=29313