X-Message-Number: 29517
From: David Stodolsky <>
Subject: Cryopreservation of Primary Porcine Hepatocytes
Date: Sun, 20 May 2007 21:28:14 +0200

--Apple-Mail-8--481368245
	delsp=yes;
	format=flowed

PhD Defendant:

Cand. med. vet. Anne-Dorthe Bonde Loven

Time and Place:

Friday 25 May 2007 at 1:30 p.m. in Lecture Hall 3-14, Thorvaldsenvej  
40,  C.

Subject Area:
Toxicology


Title of PhD Thesis:
Cryopreservation of Primary Porcine Hepatocytes


Title of Lecture:
Cryopreservation of Primary Porcine Hepatocytes


Summary:
The aim of this thesis was to compare the metabolic capacity and the  
carbohydrate metabolism in
cyopreservated porcine hepatocytes with those obtained in freshly  
isolated cells in order to examine
cryopreservated cells as a tool in the drug screening procedures.  
Cryopreservation caused a decrease
in both phase I and II metabolism. Thus, CYP2A activity in terms of  
hydroxylation of coumarin to
7-hydroxy coumarin decreased two-thirds compared to freshly isolated  
cells, whereas CYP3A activity
in terms of 6  -hydroxylation of testosterone and UGT activity in  
terms of glucuronidation of p-nitrophenol
both decreased  to about half the values in fresh cells. Carbohydrate  
metabolism was also reduced by
cryopreservation: glycogen synthesis was decreased to less than half  
and glucose produced from glyco-
genolysis and gluconeogenesis was decreased with 25-50%.
Other effects of cryopreservation included tendencies to decreased  
attachment and viability. Co-culturing
with epithelial cells did not improve the function of cryopreserved  
cells.In conclusion cryopreserved primary
porcine hepatocytes are useful in culture but have a general decrease  
of activity compared to similar fresh
hepatocytes.


Principal Supervisor:
Professor Christian Friis, Toxicology, Department of Veterinary  
Pathology, Faculty of Life Sciences, University
of Copenhagen.


Co-Supervisor:
Principal Scientist, Anette Kristensen, Novo Nordisk

Research Scientist, Birgitte Andersen, Novo Nordisk


Examination Committee:
European Scientific Consultant Nicola Hewitt, CellzDirect.

Associated Professor Dr. phil Niels Grunnet, Panum Institute,

Associated Professor Mette Tinglev Skaanild, Department of Veterinary  
Pathology, Faculty of Life Sciences,
University of Copenhagen.


--Apple-Mail-8--481368245

 Content-Transfer-Encoding: base64

[ AUTOMATICALLY SKIPPING BASE64 ENCODING! ] 

Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=29517