X-Message-Number: 30517
Date: Fri, 22 Feb 2008 14:06:59 -0500
Subject: Re: The most effective cryoprotectant is ...

   In CryoMsg 30347 Doug Skrecky wrote:

> [Lactamide has outperformed all other cryoprotectants that it has been
> tested against (see below). However at $17,390 per kilogram it is
> understandible if this cryoprotectant has never become popular. Compare
> this price to dimethylsulfoxide's price of $155 per liter.]


    Doug, both of the experiments that you cite are with
rabbit sperm, which would hardly prove that lactamide is
the most effective cryoprotectant for all mammalian
tissues, including the brain. Moreover, neither of these
experiments are vitrification experiments. Both used
classical cryobiological freezing techniques


   You do good research, but you too often generalize
far beyond what your data justifies.

              -- Ben Best

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