X-Message-Number: 3152
Date: Fri, 16 Sep 1994 22:39:20 -0400 (EDT)
From: 
Subject: SCI.CRYONICS and Amphibians.

Date sent:  16-SEP-1994 22:34:47 
>
>     Martin, I know you are a very busy person and appreciate your quick
>reply. The publications you refer to havs been posted already. I read
>those available from Current Contents and Carl. What is true for isolated
>cells in culture is true for all cells except as you have pointed out that
>the survival of an animal also depends on the anatomical structure in which
>the cells are contained. I pasted your relevent paragraph below.
>
>
>However, tissues, organs and organisms are organized at a higher level than 
 thataof individual cells.  They are complex *arrangements* of cells and the
>relationship of the cells to each other and to noncellular structures (like
>basement membrane to which capillary endothelial cells are attached) is
>critical.  So, it is not sufficient to just have the cells survive freezing, 
 the cells' relationships to each other and to structures like basement  
 membrane must also survive.
>
>     The above paragraph ALSO APPLIES to frozen amphibians. In none of the
>publication do you refer to have amphibians at -30C (under proper controlled
>conditions) been  frozen to -196C. I do not think you have answered the
>question. Nevertheless I appreciate that you have taken the time to state
>your position. Others did not even bother to respond to my question.
>     I will pursue my interest in these amphibians because my position is
>that is where answers will be found.
>
>Thank you.
>
> Jan (John) Coetzee

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