X-Message-Number: 3152 Date: Fri, 16 Sep 1994 22:39:20 -0400 (EDT) From: Subject: SCI.CRYONICS and Amphibians. Date sent: 16-SEP-1994 22:34:47 > > Martin, I know you are a very busy person and appreciate your quick >reply. The publications you refer to havs been posted already. I read >those available from Current Contents and Carl. What is true for isolated >cells in culture is true for all cells except as you have pointed out that >the survival of an animal also depends on the anatomical structure in which >the cells are contained. I pasted your relevent paragraph below. > > >However, tissues, organs and organisms are organized at a higher level than thataof individual cells. They are complex *arrangements* of cells and the >relationship of the cells to each other and to noncellular structures (like >basement membrane to which capillary endothelial cells are attached) is >critical. So, it is not sufficient to just have the cells survive freezing, the cells' relationships to each other and to structures like basement membrane must also survive. > > The above paragraph ALSO APPLIES to frozen amphibians. In none of the >publication do you refer to have amphibians at -30C (under proper controlled >conditions) been frozen to -196C. I do not think you have answered the >question. Nevertheless I appreciate that you have taken the time to state >your position. Others did not even bother to respond to my question. > I will pursue my interest in these amphibians because my position is >that is where answers will be found. > >Thank you. > > Jan (John) Coetzee Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=3152