X-Message-Number: 3166
Date: Mon, 19 Sep 1994 21:05:52 -0400 (EDT)
From: 
Subject: SCI.CRYONICS Brain Powder!

Date sent:  19-SEP-1994 21:03:29 
>Message-ID: <>
>Sender: 
>Nntp-Posting-Host: 137.53.72.136
>Organization: cell biol & anat, school of med.
>References: <>
>Date: Thu, 12 Aug 1993 00:12:24 GMT
>Lines: 28
>
>In article <>  (donald seto)
>writes:
>>what is the best way to store human tissue for extracting mRNA a year from
>>now?
>>i just popped it into liquid nitrogen...but am wondering shether there is
>>another preferred way of storing it.
>>
>
>>
>>
>>--
>>********************************************************************
>>                         
>>301-402-0580   Bldg10/Rm8D14    NIDDK/NIH    Bethesda, MD  20892
>>
>Our brain bank stores human brain samples in a frozen powder-- the tissue is
>rapidly dissected, frozed in liquid nitrogen, then powdered while still frozen
>(with a sledge hammer or in a mortar and pestle) and then stored in
>polypropylene tubes at -80 celsius.  For RNA extraction, then all you need is
>to weigh out the amount of powder you want, then throw it into your extraction
>buffer.  This gives very good quality yield of mRNA and conserves precious
>tissue.  It also makes it easier to find the tissue again a year later because
>you can keep all the tubes in one rack (small packages of tissues have a knack
>of disappering while getting shifted around in our freezer).

Item of Interest.
Jan (John) Coetzee.

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