X-Message-Number: 3166 Date: Mon, 19 Sep 1994 21:05:52 -0400 (EDT) From: Subject: SCI.CRYONICS Brain Powder! Date sent: 19-SEP-1994 21:03:29 >Message-ID: <> >Sender: >Nntp-Posting-Host: 137.53.72.136 >Organization: cell biol & anat, school of med. >References: <> >Date: Thu, 12 Aug 1993 00:12:24 GMT >Lines: 28 > >In article <> (donald seto) >writes: >>what is the best way to store human tissue for extracting mRNA a year from >>now? >>i just popped it into liquid nitrogen...but am wondering shether there is >>another preferred way of storing it. >> > >> >> >>-- >>******************************************************************** >> >>301-402-0580 Bldg10/Rm8D14 NIDDK/NIH Bethesda, MD 20892 >> >Our brain bank stores human brain samples in a frozen powder-- the tissue is >rapidly dissected, frozed in liquid nitrogen, then powdered while still frozen >(with a sledge hammer or in a mortar and pestle) and then stored in >polypropylene tubes at -80 celsius. For RNA extraction, then all you need is >to weigh out the amount of powder you want, then throw it into your extraction >buffer. This gives very good quality yield of mRNA and conserves precious >tissue. It also makes it easier to find the tissue again a year later because >you can keep all the tubes in one rack (small packages of tissues have a knack >of disappering while getting shifted around in our freezer). Item of Interest. Jan (John) Coetzee. Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=3166