X-Message-Number: 3172 From: Date: Thu, 22 Sep 94 19:57:35 EDT Subject: SCI. CRYONICS latest Ukrainian Today we have the email report of Dr. Pichugin and Dr. Zhegunov on the 1st part of the 4th stage of the sheep head work--not in time for the October IMMORTALIST. The full report--along with the following one, if we have it then, and some of the photos, will appear in the Novermber IMMORTALIST (published in October). Tonight I'll just give a very brief second-hand report. In general, the report was very encouraging. Cracking was NOT observed at the naked eye level or the light microscope level, and was not mentioned at the electron microscope level. This is in contrast to results in animals which were treated by methods similar to those used for human patients in the past, developed by Jerry Leaf, Mike Darwin, Hugh Hixon, and others--these showed serious cracking at all levels, if I read correctly the reports kindly furnished by Mike Darwin. Furthermore, "the structure of the thawed glycerolized tissues was very good." Pyramidal neuronal axons and dendrites have no ruptures or other mechanical injuries [in the photo being discussed]. Under the electron microscope there was no drastic alteration of the cytoplasmic organelles, although there was "increased vacuolization of the cytoplams and some changes in the mitochordrial crysts." There is much more, and some of it is unclear, at least to me, and even when the meaning is clear the interpretation is not always obvious. For a definitive interpretation, we will of course have to wait until several people have had an opportunity to read the reports in full, see all the photos, get answers to questions that arise as to language or fact or procedure, and compare to the Darwin/Leaf/Hixon reports and photos. With fingers crossed, however, I am considerably enouraged that the Cryonics Institute patients treated by these methods are in relatively good condition, both absolutely and by comparison to those treated by other methods. But even at best we have a lot more work to do, and we intend to get on with it, trying to benefit from all inputs. Robert Ettinger Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=3172