X-Message-Number: 32002
Date: Thu, 24 Sep 2009 16:34:39 -0700
Subject: Re: John Clark's CAS question
From: Jeff Davis <>

John,
I think I posted these two documents to the cryonet.

http://docs.google.com/Doc?id=dnpz8m3_0f2d4r7fz&btr=EmailImport

As you will see by reading them, the first reports the result of the
autotransplant of whole monkey ovaries.  Worth noting is that the
ovaries had been frozen using the CAS method and without
cryoprotectants, and that they had been stored in LN2 for a month
before being re-implanted.

The ovaries then resumed some degree of function(howbeit with a bit of
prodding), as indicated by the resumption of menstrual cycles.

The second document reports on pig ovaries preserved by the CAS method
for one week at LN2 temps, and then thawed and examined.  Follicle
preservation by this method increased to 17% from 3%.  The researchers
also report less interstitial damage in the tissues than by alternate
methods of cryopreservation.

                          **********************************************

There was some discussion of a glass of water being frozen without the
usual volumetric expansion, and speculation on what was going on with
this, particularly with the possibility that the water was actually
being vitrified.  This seems highly unlikely, if not barred outright
by the laws of physics.

More to the point, that aspect of the CAS discussion was a
distraction.  Forget the glass of distilled water. Who cares?  We need
to focus on how the CAS method effects biological specimens.

                           ************************************************

I'll continue to inquire of the researchers working with this tech
regarding their results, and post what I find to the cryonet.

Best, Jeff Davis

 "Everything's hard till you know how to do it."
                     Ray Charles



> I've been hearing a lot on Cryonet about this CAS freezer stuff, and
> it would certainty be wonderful if true, but can anybody find an
> indication that one word of what they are typing onto websites is
> true? I can't. I know how to type too, but that doesn't mean
> everything I type is true.
>
>  John K Clark
>

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