X-Message-Number: 32566 Date: Thu, 22 Apr 2010 21:22:44 -0700 (PDT) From: Subject: could adzucki beans explain why Asians have less hair greying... Biosci Biotechnol Biochem. 2005 May;69(5):873-82. Hot-water extracts from adzuki beans (Vigna angularis) stimulate not only melanogenesis in cultured mouse B16 melanoma cells but also pigmentation of hair color in C3H mice. Itoh T, Furuichi Y. Imuraya Confectionary Co., Ltd., Mie, Japan Abstract A hot-water extract of adzuki was obtained by boiling beans of adzuki (Vigna angularis). This hot-water extract was fractionated using HP-20 column chromatography. Its distilled water fraction (WEx) was found to stimulate tyrosinase activity in cultured mouse B16 melanoma cells and hair color pigmentation in C3H mice. At concentrations of 1-3 mg/ml, WEx stimulated melanogenesis without inhibiting cell growth. During this effect, WEx activated tyrosinase-inducing activity in the cells, but did not activate tyrosinase, which exists at an intracellular level. In this study, WEx increased cyclic adenosine-3',5'-monophospate (cAMP) content in the cells and protein kinase A (PKA) activity, and stimulated translocation of cytosolic protein kinase C (PKC) to the membrane-bound PKC. These results suggest that the addition of WEx activates the adenylcyclase and protein kinase pathways and, as a result, stimulates melanogenesis. WEx was found to have pigmentation activity on hair color in C3H mice. It might be useful in anti-graying, protecting human skin from irradiation. PMID: 15914904 Free text> http://www.jstage.jst.go.jp/article/bbb/69/5/873/_pdf Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=32566