X-Message-Number: 32693 From: Date: Thu, 8 Jul 2010 17:25:03 EDT Subject: Rejuve without cryo? Hi all: The cryo scenario has always been die, get frozen, get thawed, have brain put into new young body. But recent progress in growing organs suggests the interesting possibility of skipping all steps but the last. Maybe we can be rejuved while we still live. The Wall St Journal just reported researchers have created a functional rat lung from a cadaver-lung-scaffolding plus stem cells. Recently, others have done a beating heart, a liver, bladders, corneas and more. It appears the organ making business is going very well. (_http://online.wsj.com/article/SB10001424052748704227304575326913608488480.html_ (http://online.wsj.com/article/SB10001424052748704227304575326913608488480.html) ) The cryonics idea is to preserve the brain in the hope it can later be restored and put into a new, young healthy body. We always assumed it would be a hundred years before the body was possible. But at the rate they are going, they may be able to give us significant new organs in ten years, and maybe a whole new collection of organs (AKA "a body") in twenty or thirty. This is very good news for cryonics. Indeed, even without cryonics, some of us might live to have our brain plugged into a new body, "from warm to warm". There has been no research in whole-brain transplants, but in the 1950's the Russians transplanted a head, which lived for a while. (One dog, two heads. At the time we thought it was pointless and cruel and typical of mad communist scientists, like something out of Gulliver's Travels. But now it's more interesting.) Later someone did a monkey head. Perhaps we could grow a new body, transplant the old head and spinal cord, and get the peripheral nerves to connect, maybe with the help of stem cells. Or maybe we don't need a complete body. Everyone assumes a complete new body with an old brain would be youthful, because all the organs would be new. But maybe we need only one or two regrown organs. There is support for this, because long ago researchers attached the arteries of young and old rats and the old rats lived about twice as long. They even did a two stage experiment where they let the young rat grow old and then replaced it with a new young rat -- and, as you might expect, the old rat lived three times as long as usual. So some substances in the blood of the young rat let the old body be healthy. (Just like a vampire movie -- I *knew* those Twilight kids were on to something! ) So we don't need a new body or organs, just new blood -- or some of its components. (Joining bloodstreams is called "parabiosis", but if you google it don't be put off by the fact it's also used by new-agers for one of their doctrines. I have a paper copy of the paper from the 50's but can't find an online reference.) More recently someone replaced the ovaries of an old rat with those of a young one, and restored youth and longevity. (_http://www.eurekalert.org/pub_releases/2010-06/esoh-otr062810.php_ (http://www.eurekalert.org/pub_releases/2010-06/esoh-otr062810.php) or see copy below) Hmm... how would this work for a man? :-) We might suppose that most of the organs of an old individual work well, and there are only a few that put things into the blood. (I.e. muscles, skin and eyes have other functions, while organs like the liver, pancreas and thyroid make hormones and do chemistry.) So if we replaced just one or two organs that might have the same effect. (Or if we found the substances and injected them.) This is interesting because they've regrown mouse livers (sort-of) already: _http://www.encyclopedia.com/doc/1G1-154174178.html_ (http://www.encyclopedia.com/doc/1G1-154174178.html) Plus, you can remove part of the liver and have it regrow, and maybe the new part acts young. Assuming that in 5-10 years they can regrow most organs, we might just grow a new liver and transplant it, and rejuvenate the subject. We might never need cryonics at all, nor brain transplants. I never thought about this before, because ten years ago there was no promise of growing organs. I did correspond with some people about parabiosis and blood factors, but whole organs seemed impossible. Alan Ovarian transplantation restores fertility to old mice and also lengthens their lives Rome, Italy: Scientists have discovered that when they transplant ovaries from young mice into aging female mice, not only does the procedure make the mice fertile again, but also it rejuvenates their behaviour and increases their lifespan. The question now is: could ovarian transplants in women have the same effect? Dr Noriko Kagawa will tell the 26th annual meeting of the European Society of Human Reproduction and Embryology in Rome today (Tuesday) that successful ovarian transplants increased the lifespan of the mice by more than 40%. "At present ovarian transplants are performed with the aim of preserving a woman's fertility after cancer treatment for instance, or of extending her reproductive lifespan. However, the completely unexpected extra benefit of fertility-preserving procedures in our mouse studies indicates that there is a possibility that carrying out similar procedures in women could lengthen their lifespans in general," she said. A very small number of women in the world have had ovarian transplants, and some have been more successful than others. Dr Kagawa stressed that there was still a lot of research to be carried out before it would be known whether ovarian transplants had similar, rejuvenating effects in women, particularly as it would involve waiting for many years until the patients became older. Dr Kagawa, Associate Director for Research at the Kato Ladies' Clinic in Tokyo (Japan), told the conference that she and her colleagues had conducted two mouse experiments. In the first, both ovaries were removed from young female mice (about 140 days old), and transplanted in to six older mice (aged over 525 days) that were too old to be fertile any more. In the second experiment, only one ovary was removed from the young mice (about 170 days old) and transplanted into eight aged mice (over 540 days old). The average normal lifespan for this particular breed of mice (C57BL/6J) is 548 days, and they normally reach a mouse "menopause" at about 525 days old. All the mice that received transplants in both experiments became fertile again, while control mice that had not received transplants did not. In the first experiment the mice resumed normal reproductive cycles that lasted for more than 80 days, and in the second experiment, they lasted for more that 130 days. Dr Kagawa said: "All the mice in both experiments that had received transplants resumed the normal reproductive behaviour of young mice. They showed interest in male mice, mated and some had pups. Normally, old mice stay in the corner of the cage and don't move much, but the activity of mice that had had ovarian transplants was transformed into that of younger mice and they resumed quick movements. Furthermore, the lifespan of the mice who received young ovaries was much longer than that of the control mice: the mice that had received two ovaries lived for an average of 915 days, and the mice that had received one ovary, for an average of 877 days. The newest of our data show the life span of mice that received transplants of young ovaries was increased by more than 40%. "The results show that transplanted normal ovaries from young mice can function in old, infertile mice, making them fertile again, but, in addition, extending their lifespan. Women who have ovarian tissue frozen at young ages, perhaps because they are about to embark on cancer treatment, can have their young ovarian tissue transplanted back when they are older. Normally we would be doing this simply to preserve their fertility or to expand their reproductive lifespan. However, our mice experiment suggests that this might also improve overall longevity. Further research has to be conducted before we can know whether or not this is the case." Dr Kagawa said it was not known why the ovarian transplant increased the lifespan of the mice, but it might be because the transplants were prompting the continuation of normal hormonal functions. She and her colleagues have been collaborating for the past six years with Dr Sherman Silber, from St Luke's Hospital, in St Louis, Missouri (USA), who has performed a number of successful ovarian transplants in women, either because they were about to be treated for cancer or because they had not yet found the right partner in life. Their future collaborative research will include investigating whether it is possible for a woman to have a transplant using an ovary that is not her own and with minimal drugs to suppress the body's natural immune response to what it perceives as a "foreign" body. They are also looking at culturing follicles in ovarian tissue in the laboratory in order to obtain mature eggs that can be used for IVF. In the meantime, the researchers believe it is very important for doctors and patients to know that women have options when faced with cancer treatment that could destroy their fertility. 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