X-Message-Number: 32897
Date: Fri, 1 Oct 2010 21:43:54 -0700 (PDT)
From: 
Subject: Cryopreservation of periodontal ligament cells with magnetic ...

Cellular viability was 96% for the most effective magnetic protocol.

Cryobiology. 2010 Aug;61(1):73-8. Epub 2010 May 15.

Cryopreservation of periodontal ligament cells with magnetic field for tooth 
banking.

Kaku M, Kamada H, Kawata T, Koseki H, Abedini S, Kojima S, Motokawa M, Fujita T,
Ohtani J, Tsuka N, Matsuda Y, Sunagawa H, Hernandes RA, Ohwada N, Tanne K.

Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima 
University Graduate School of Biomedical Science, 1-2-3 Kasumi, Minami-ku, 
Hiroshima 734-8553, Japan.
Abstract

The purpose of this study was to establish a long-term tooth cryopreservation 
method that can be used for tooth autotransplantation. Human periodontal 
ligament (PDL) cells were frozen in 10% dimethyl sulfoxide (Me(2)SO) using a 
programmed freezer with a magnetic field. Cells were cryopreserved for 7 days at
-150 degrees C. Immediately after thawing, the number of surviving cells was 
counted and the cells were cultured; cultured cells were examined after 48 h. 
Results indicated that a 0.01 mT of a magnetic field, a 15-min hold-time, and a 
plunging temperature of -30 degrees C led to the greatest survival rate of PDL 
cells. Based on these findings, whole teeth were cryopreserved under the same 
conditions for 1 year. The organ culture revealed that the PDL cells of 
cryopreserved tooth with a magnetic field could proliferate as much as a fresh 
tooth, although the cells did not appear in the cryopreserved tooth without a 
magnetic field. Histological examination and the transmission electron 
microscopic image of cryopreserved tooth with a magnetic field did not show any 
destruction of cryopreserved cells. In contrast, severe cell damage was seen in 
cells frozen without a magnetic field. These results indicated that a magnetic 
field programmed freezer is available for tooth cryopreservation.
PMID: 20478291 [PubMed - in process]

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