X-Message-Number: 33099 Date: Sun, 05 Dec 2010 09:06:00 +0100 From: "Hal Tindale" <> Subject: parallel histories theory --========GMXBoundary106701291536360921414 imagine a long beach with a hundred houses full of surfers. they have never met but they all go surfing at the same time. someone is going to get a surfboard in the back of the head - hal ----- Original Message ----- From: CryoNet Sent: 12/04/10 09:00 PM To: Subject: CryoNet #33097 CryoNet - Sat 4 Dec 2010 #33097: could argon/xenon improve vitrification solutions? [oberon] Rate This Digest: http://www.cryonet.org/cgi-bin/rate.cgi?msg=33097%2D33097 Administrivia To subscribe to CryoNet, send email to: with the subject line (not message _body_): subscribe To unsubscribe, use the subject line: unsubscribe To post a message to CryoNet, send your message to: from the same address to which you are aubscribed. Send questions, comments, or feedback to with "CryoNet" or "cryonics" somewhere in the Subject line. ---------------------------------------------------------------------- Message #33097 Date: Fri, 3 Dec 2010 09:28:32 -0800 (PST) From: Subject: could argon/xenon improve vitrification solutions? [Argon and xenon, offer powerful neuroprotectation, and act as novel cryoprotectants. Could argon/xenon reduce the toxicity of vitrification solutions? By preforming an intracellular clathrate, cell dehydration and membrane rupture are largely avoided during both freezing and rewarming. Clathrate would also presumably reduce vitrification solution toxicity, by limiting intracellular egress of toxic solvents like DMSO, and ethylene glycol. IMHO, there are enough unused novel technologies around such as directional freezing, variable magnetic field freezing, flavonoid additives, and here clathrate formation for cryonics to develop fully reversible whole body cryopreservation protocols within 5 years. As far as I am aware, the only limiting factor here preventing this is a lack of vision, and more importantly alas a lack of money. It is hard to say how to get around the funding desert that surrounds both cryonics, and organ cryopreservation. A vastly more effective sales strategy is here indicated than I have seen to date. Perhaps "paper" research into novel technologies, and development of a shared library of all the relevant research papers published on these novel technologies would be a start. Perhaps a think tank with input from both professional salesman and skeptical scientists could then be used to develop an effective sales campaign. Otherwise it could easily take over 50 years before the technologies available now are finally used to engineer fully reversible whole organ (and whole body) cryopreservation.] J Huazhong Univ Sci Technolog Med Sci. 2007 Aug;27(4):426-8. Experimental study on the cryopreservation of LLC-PK1 epithelial cells with hypoxic UW solution. Wan C, Wang C, Liu T, Wang H, Yang Z. Department of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. Abstract The effects of oxygen partial pressure on cryopreservation of the cells with organ preservation solution were explored. Hypoxic UW solution was made by purging the UW solution with argon. The pig proximal tubule epithelial cells (LLC-PK1 cells) were cryopreserved in hypoxic UW solution (Ar-UW group) or standard UW solution (UW group) at 4 degrees C for 48 h. Trypan blue staining and LDH detection were performed to evaluate the injury of the cells. The results showed that the oxygen partial pressure in Ar-UW group was significantly declined from 242+/-6 mmHg to 83+/-10 mmHg. After cryopreservation at 4 degrees C for 48 h, LDH leakage rate and Trypan blue-stained rate in Ar-UW group were (11.3+/-3.4)% and (10.5+/-4.7)%, respectively, which were significantly lower than in UW group [(49.5+/-6.9)% and (47.6+/-9.3)% respectively, both P<0.01]. It was concluded that lower oxygen partial pressure of UW solution was more beneficial to the cryopreservation of LLC. PMID: 17828502 [Here xenon forms an intracellular clathrate at above freezing temperatures, and thereby prevents most cell dehydration during water ice formation at sub-zero temperatures. This proved to be highly effective at preserving cell viability by preventing membrane rupture by large ice crystals, particularly during rewarming. Unlike pure water ice, the xenon/water clathrate itself is apparently non-damaging.] Int J Clin Exp Pathol. 2008 Jan 1;1(5):440-7. Cardiac mitochondrial membrane stability after deep hypothermia using a xenon clathrate cryostasis protocol - an electron microscopy study. Sheleg S, Hixon H, Cohen B, Lowry D, Nedzved M. Innovative Biological Preservation Technologies LLC Scottsdale, AZ, USA. Sergey. Abstract We investigated a new cryopreservation method using xenon, a clathrate-forming gas, under medium pressure (100psi). The objective of the study was to determine whether this cryostasis protocol could protect cardiac mitochondria at cryogenic temperatures (below 100 degrees Celsius).We analyzed transmission electron microscopy images to obtain information about changes in mitochondrial morphology induced by cryopreservation of the hearts. Our data showed absence of mitochondrial swelling, rupture of inner and outer membranes, and leakage of mitochondrial matrix into the cytoplasm after applying this cryostasis protocol. The electron microscopy results provided the first evidence that a cryostasis protocol using xenon as a clathrate-forming gas under pressure may have protective effects on intracellular membranes. This cryostasis technology may find applications in developing new approaches for long-term cryopreservation protocols. PMID: 18787624 Free text> http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2480575/pdf/ijcep0001-0440.pdf Ann Neurol. 2010 Mar 8. [Epub ahead of print] Xenon enhances hypothermic neuroprotection in asphyxiated newborn pigs. Chakkarapani E, Dingley J, Liu X, Hoque N, Aquilina K, Porter H, Thoresen M. Department of Clinical Sciences at South Bristol, University of Bristol, Bristol, United Kingdom. Abstract OBJECTIVE: To investigate whether inhaling 50% xenon during hypothermia (HT) offers better neuroprotection than xenon or HT alone. METHODS: Ninety-eight newborn pigs underwent a 45-minute global hypoxic-ischemic insult severe enough to cause permanent brain injury, and 12 pigs underwent sham protocol. Pigs then received intravenous anesthesia and were randomized to 6 treatment groups: (1) normothermia (NT; rectal temperature 38.5 degrees C, n = 18); (2) 18 hours 50% xenon with NT (n = 12); (3) 12 hours HT (rectal temperature 33.5 degrees C, n = 18); (4) 24 hours HT (rectal temperature 33.5 degrees C, n = 17); (5) 18 hours 50% xenon with 12 hours HT (n = 18); and (6) 18 hours 50% xenon with 24 hours HT (n = 17). Fifty percent xenon was administered via a closed circle with 30% oxygen and 20% nitrogen. After 10 hours rewarming, cooled pigs remained normothermic until terminal perfusion fixation at 72 hours. Global and regional brain neuropathology and clinical neurological scor es were performed. RESULTS: Xenon (p = 0.011) and 12 or 24 hours HT (p = 0.003) treatments offered significant histological global, and regional neuroprotection. Combining xenon with HT yielded an additive neuroprotective effect, as there was no interaction effect (p = 0.54). Combining Xenon with 24 hours HT offered 75% global histological neuroprotection with similarly improved regional neuroprotection: thalamus (100%), brainstem (100%), white matter (86%), basal ganglia (76%), cortical gray matter (74%), cerebellum (73%), and hippocampus (72%). Neurology scores improved in the 24-hour HT and combined xenon HT groups at 72 hours. INTERPRETATION: Combining xenon with HT is a promising therapy for severely encephalopathic infants, doubling the neuroprotection offered by HT alone. ANN NEUROL 2010. PMID: 20658563 [PubMed - as supplied by publisher] Stroke. 2008 Apr;39(4):1307-13. Epub 2008 Feb 28. Xenon and hypothermia combine additively, offering long-term functional and histopathologic neuroprotection after neonatal hypoxia/ischemia. Hobbs C, Thoresen M, Tucker A, Aquilina K, Chakkarapani E, Dingley J. Department of Clinical Sciences at South Bristol, University of Bristol, Bristol, UK. Abstract BACKGROUND AND PURPOSE: Hypoxic/ischemic (HI) brain injury affects 1 to 6 per 1000 live human births, with a mortality of 15% to 20%. A quarter of survivors have permanent disabilities. Hypothermia is the only intervention that improves outcome; however, further improvements might be obtained by combining hypothermia with additional treatments. Xenon is a noble anesthetic gas with an excellent safety profile, showing great promise in vitro and in vivo as a neuroprotectant. We investigated combinations of 50% xenon (Xe(50%)) and hypothermia of 32 degrees C (HT(32 degrees C)) as a post-HI therapy. METHODS: An established neonatal rat HI model was used. Serial functional neurologic testing into adulthood 10 weeks after injury was performed, followed by global and regional brain histopathology evaluation. RESULTS: In the combination Xe(50%)HT(32 degrees C) group, complete restoration of long-term functional outcomes was seen. Hypothermia produced improvement on short- (P<0.00 1) and long- (P<0.001) term functional testing, whereas Xe(50%) alone predominantly improved long-term function (P<0.05), suggesting that short-term testing does not always predict eventual outcome. Similarly, the Xe(50%)HT(32 degrees C) combination produced the greatest (71%) improvement in global histopathology scores, a pattern mirrored in the regional scores, whereas Xe(50%) and HT(32 degrees C) individually produced smaller improvements (P<0.05 and P<0.001, respectively). The interaction between the 2 treatments was additive. CONCLUSIONS: The xenon/hypothermia combination additively confers greater protection after HI than either treatment alone. The functional improvement is almost complete, is sustained long term, and is accompanied by greatly improved histopathology. The unique safety profile differentiates xenon as an attractive combination therapy with hypothermia to improve the otherwise bleak outcome from neonatal HI. PMID: 18309163 Free text> http://stroke.ahajournals.org/cgi/reprint/39/4/1307 Crit Care. 2009;13(6):R206. Epub 2009 Dec 17. Argon: neuroprotection in in vitro models of cerebral ischemia and traumatic brain injury. Loetscher PD, Rossaint J, Rossaint R, Weis J, Fries M, Fahlenkamp A, Ryang YM, Grottke O, Coburn M. Department of Anesthesiology, University Hospital of the RWTH Aachen, Aachen, Germany. Comment in: Crit Care. 2010;14(1):117. Abstract INTRODUCTION: Recently, it has been shown in several experimental settings that the noble gases xenon and helium have neuroprotective properties. In this study we tested the hypothesis that the noble gas argon has a neuroprotective potential as well. Since traumatic brain injury and stroke are widespread and generate an enormous economic and social burden, we investigated the possible neuroprotective effect in in vitro models of traumatic brain injury and cerebral ischemia. METHODS: Organotypic hippocampal slice cultures from mice pups were subjected to either oxygen-glucose deprivation or to a focal mechanical trauma and subsequently treated with three different concentrations (25, 50 and 74%) of argon immediately after trauma or with a two-or-three-hour delay. After 72 hours of incubation tissue injury assessment was performed using propidium iodide, a staining agent that becomes fluorescent when it diffuses into damaged cells via disintegrated cell membranes. RESULTS: We could show argon's neuroprotective effects at different concentrations when applied directly after oxygen-glucose deprivation or trauma. Even three hours after application, argon was still neuroprotective. CONCLUSIONS: Argon showed a neuroprotective effect in both in vitro models of oxygen-glucose deprivation and traumatic brain injury. Our promising results justify further in vivo animal research. PMID: 20017934 Free text> http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2811924/pdf/cc8214.pdf Neurosci Lett. 2009 Sep 4;460(3):232-6. Epub 2009 Jun 7. Neuroprotection (and lack of neuroprotection) afforded by a series of noble gases in an in vitro model of neuronal injury. Jawad N, Rizvi M, Gu J, Adeyi O, Tao G, Maze M, Ma D. Department of Anaesthetics, Pain Medicine and Intensive Care, Imperial College London, Chelsea and Westminster Hospital, 369 Fulham Road, London SW10 9NH, United Kingdom. Abstract Xenon-induced neuroprotection has been well studied both in vivo and in vitro. In this study, the neuroprotective properties of the other noble gases, namely, krypton, argon, neon and helium, were explored in an in vitro model of neuronal injury. Pure neuronal cultures, derived from foetal BALB/c mice cortices, were provoked into injury by oxygen and glucose deprivation (OGD). Cultures were exposed to either nitrogen hypoxia or noble gas hypoxia in balanced salt solution devoid of glucose for 90min. The cultures were allowed to recover in normal culture medium for a further 24h in nitrogen or noble gas. The effect of noble gases on cell reducing ability in the absence of OGD was also investigated. Cell reducing ability was quantified via an MTT assay and expressed as a ratio of the control. The OGD caused a reduction in cell reducing ability to 0.56+/-0.04 of the control in the absence of noble gas (p<0.001). Like xenon (0.92+/-0.10; p<0.001), neuroprotection was afforded by argon (0.71+/-0.05; p<0.01). Neon and krypton did not have a protective effect under our experimental conditions. Helium had a detrimental effect on the cells. In the absence of OGD, krypton reduced the reducing ability of uninjured cells to 0.84+/-0.09 (p<0.01), but argon showed an improvement in reducing ability to 1.15+/-0.11 (p<0.05). Our data suggest that the cheap and widely available noble gas argon may have potential as a neuroprotectant for the future. PMID: 19500647 Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=33097 ---------------------------------------------------------------------- End of CryoNet Digest ********************* --========GMXBoundary106701291536360921414 Content-Type: text/html; charset="utf-8" [ AUTOMATICALLY SKIPPING HTML ENCODING! ] Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=33099