X-Message-Number: 33127 From: Date: Wed, 22 Dec 2010 17:33:44 -0500 (EST) Subject: Achievements A prime rule of political discussion is not to let the opposition set the agenda. Without being dishonest, keep the focus on the positive. As time and energy permit, and hoping others will help out, I intend to point out to newcomers, and remind old timers, of our very considerable achievements, in particular at the Cryonics Institute (CI) and Alcor. Segments will vary in topic and there will be some repetition, which is always necessary. Certainly the cryonics movement is still small, and there have been many failures and errors, beginning with my initial unrealistic optimism about the rate of growth and acceptance. Nevertheless, growth continues--members and patients--and at improved rates in relatively recent years. World wide, but mostly at CI and Alcor, members number roughly 2,000 and patients 212. More importantly, many of us now have relatives and friends in cryostasis, with whom we hope one day to be reunited, a very big plus. Today let's put the spotlight on improvements in procedures at CI and Alcor. In the earliest years about all we could do was read the cryobiological literature and attempt, with available personnel and resources, to approximate for our patients the procedures that seemed to work best. There was necessarily a lot of guesswork, since the literature did not deal with human bodies or brains, but there were quite a few reports in the journals about the effects on mammals of post mortem warm ischemia and related matters. (Some of those reports, which I'll cite by and by, provide reason for a degree of optimism.) The main thing I want to say or reiterate today is that, both at CI and Alcor, there has always been a keen awareness of the need for experimental verification of the efficacy of our procedures. Obtaining the breadth and depth of verification desired was and is a daunting job, but we tried and to some extent succeeded. Alcor used light and electron microscopy to demonstrate that patients or animal subjects, back when they relied on glycerol, experienced much less damage than controls that were straight frozen. CI, led by professional cryobiologist Dr. Yuri Pichugin, using sheep heads, rabbit brain pieces, and rat brain pieces, showed that our vitrification procedure retained not only structure but function, as evidenced by the K/Na (potassium/sodium) ratio criterion, believed to be a broad marker of viability. Before that, using sheep heads and with the collaboration of colleagues, Dr. Pichugin showed that the older CI procedures using gylycerol provided definitely better results than straight freezing. The problems now include application to whole brains, and some of these problems, under Aschwin and Chana de wolf, are being subsidized by the Immortalist Society, which shares a roof with the Cryonics Institute. Note on animal research: CI and Dr. Pichugin have never caused experimental animals to suffer. When live animals were used, mostly rats, they were first anaesthetized and then euthanized. Robert Ettinger Content-Type: text/html; charset="US-ASCII" [ AUTOMATICALLY SKIPPING HTML ENCODING! ] Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=33127