X-Message-Number: 33385
From: 
Date: Mon, 28 Feb 2011 02:56:51 EST
Subject: Melody Maxim's Distorted Reality 18

Content-Language: en

 
 
You write:   
The only way to "assume a working knowledge of perfusion at Board  

Certification level," is to hire properly educated and trained perfusionists. SA
and Alcor can certainly afford to hire qualified perfusionists, (or at least  
retain a small pool, to rotate call), but I'm not sure CI can do this. If 
not,  they must work with what they have.  
I couldn't agree more with the caveat (to which I gather you do not  
subscribe) that cryonics is a discipline within medicine that requires  

specialized mentoring and training. A cardiothoracic surgeon is NOT competent to
do 
perfusion without extensive training in perfusion; particularly training to  
develop split second judgment, rapid responses and a complete awareness of 
the  circuit he is operating: as if it were an extension of his body. People 
who know  how to drive well have a constant awareness of the position and 
likely behavior  of the other drivers around them; they are constantly alert 
to likely (and  unlikely) deviations in other drivers' behavior, as well as 
in road conditions,  and the behavior of their own automobile.  
When I am in an OR I can immediately sense a wide range of things  being 
right or being amiss; I know the normal sound of a ventilator cycling  

properly, of roller pumps or centrifugals operating smoothly, of the appropriate
level of tension in the voices of every person in theatre, of the sound of a  
pump that has run dry or has an occluded line; the sound of bearing 

starting to  fail on a pump, the sound of an uncoupled impeller on a centrifugal
pump... You  don't get these things in a day or a week, and if you can't get 
them at all then  DO NOT DO COMPLEX PROCEDURES that can deprive a patient of 
cryoprotection.  Nothing that CI can add to their technological base will 
undo the harm of a  patient who is massively air embolized and cannot be 

cryoprotected as a  consequence. This has happened at Alcor again and again...
Alcor had a perfusionist for a year or so to whom I carefully  explained 
that in patients who have had substantial cold ischemia, the aorta  will be 
under very slight negative pressure. This is totally counterintuitive  unless 
you understand the pathophysiology of ischemia. Absent ion pumping, large  
amounts of vascular water move into the cells under the influence of the  
Gibbs-Donan effect. Every bit of mechanically and osmotically accessible  
vascular water will end up trans-located into the intracellular compartment.  
Starting about 15 min post arrest the CVP actually goes slightly negative (if  
you have a highly sensitive and properly zeroed pressure transducer to 

measure  it). When you do a median sternotomy on such patients the evidence that
the  vascular volume has been depleted is that the aorta is concave. The 
aortas  normal configuration, if cut out of the body and placed on a table top 
or in a  basin of liquid, is NOT concave it is cylindrical. Why is this? The 
short answer  is because the aorta has elastic fibers arranged in such a 
way as to make it  cylindrical. The take home message is that the instant you 
make your aortotomy  with that #11 blade in a concave aorta air will enter 
the aortic root/arch: air  which you cannot see and which it is virtually 
impossible to evacuate because of  the physical structure of the vessel. The 
first time Jerry Leaf and I saw a  concave aorta we were smart enough to 
realize that we needed to do something;  but it took us about 10 minutes to 

figure out what to do; should we cannulate a  femoral vessel and pressurize the
aorta? That would take a lot of time, but  would certainly work. We 
ultimately decided to fill the thorax with perfusate  and do the aortotomy and 

cannula insertion under water. We were smart enough to  identify that truly 
unique 
to cryonics problem and deal with it. Unfortunately,  even after being 
cautioned about this, the perfusionist that Alcor had on staff  paid no heed 
with the expected results.  
What I do have is 30 years of experience doing perfusion under  demanding 
conditions that most perfusionists will never encounter. And I am not  

speaking of cryonics cases, but rather of the years of research work with models
such as Peter Safar's dog model of CPB reperfusion after many minutes of 
global  normothermic ischemia. I have trained skilled perfusionists in this 
model and  they do not learn it easily; one described it as a combination of 
his worst  perfusion experiences all rolled into one; no volume, enormous 
demand for flow,  sky high CVP, severe acidosis, totally deranged blood gases, 
no SVR and then, in  seconds, massive SVR as a result of vasopressin and epi 
administration; and then  almost instantly a tight venous reservoir bag 

which required draining into  accessory reservoirs the volume of which could not
be returned to the animals  for HOURS after CPB was over!  
Below are photos taken during one of these experiments (circa  1998). Most 
of the equipment you see was owned by me and maintained by me and I  could 
reliably operate all of it:  
I could also manage intensive care of the animals (including vent  

management and set-up and basic servicing of the vents of we used). That  
expertise 
extended to a wide range of ancillary equipment; IV pumps, syringe  pumps, 
gas blenders, anesthesia machines (everything from tearing down a  vaporizer 
to reworking gas regulators). The only thing I couldn't operate in the  
pictures above was the computer control system; we were in the process of  

automating CPB and Brian Wowk was the only one who understood that hardware and
software.  
ICU care was extensive for all of our models (TBW and  
ischemia-reperfusion) and we had to take a 12 hour shift alone:  


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