X-Message-Number: 33432
Date: Sun, 6 Mar 2011 16:18:26 -0700
Subject: custom manufactured liposomes
From: Jeff Davis <>

I've occasionally posted what I think might prove a successful
approach to achieving low cellular damage and low toxicity
cryopreservation -- essentially a cryopreservation good enough so that
only the rewarming challenge remains as the last obstacle to
reversible suspension.  A crucial element of that process would be the
perfection of a liposome-mediated cryoprotectant delivery system.
This would consist of a family of manufactured liposomes each targeted
for binding to a particular cell type by the appropriate complementary
surface-membrane protein.  Each tissue-specific liposome would contain
a cryoprotectant mix customized to optimally protect that particular
cell type. And each liposome would have surface trans-membrane protein
structures designed -- or copied from naturally occurring liposome
docking mechanisms -- to effect the injection of the cryoprotectant
across the target cell membrane.  This method of administering
cryoprotectant -- as an alternate to the blunt instrument of gross,
whole organism, "vanilla" cryoprotectant perfusion that relies on cell
penetration by simple diffusion -- will finally allow the use of more
effective and less toxic cryoprotectant mixes (trehalose as one
component is the first that comes to mind, but there are many) which
have up till now been unusable because of inadequate rates of
trans-membrane diffusion. .

I suggested this approach years ago, and have been watching since for
the commercial development of custom liposome manufacture.  Well, here
it is:

Microfluidic Assembly Line for mass producing synthetic cell-like compartments



http://nextbigfuture.com/2011/03/microfluidic-assembly-line-for-mass.html?utm_source=feedburner&utm_medium=feed&utm_campaign=Feed%3A+blogspot%2Fadvancednano+%28nextbigfuture%29

Combining this tech with the new CAS freezing method,... well, I'd
like to see some experimentation with lab mice, or maybe fish, or
chicken.

Wouldn't you?

Best, Jeff Davis

              "Everything's hard till you know how to do it."
                                                   Ray Charles

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