X-Message-Number: 3882 Date: Wed, 22 Feb 1995 11:54:14 GMT From: (Michael Clive Price) Subject: Sternberg article SCI.CRYONICS My thanks to Art Quaife for passing on Hal Sternberg's repsonse to my query about cellular degeneration during storage at approximately -80C. > Based on muscle cell contractility after freezing and thawing, > and the literature on cell freezing, Hal believes that storage at > above -80 degrees C should be for no more than 72 hours. This seems a rather short period. How does this square with the experiments of Suda et al [*] who demonstrated recovery of cortical functions from storage of up to 7 years at -20C (and perhaps -60C, the articles are ambiguous on the lower temperature)? This point of of especial significance to non-US cryonics patients who get flown to the US on dry-ice. [*] Isamu Suda, Kyoko Kito and Chizuko Adachi _Viability of Long Term Frozen Cat Brain in vitro_ in Nature, Vol 212, 268-270 (1966) and _Bioelectric discharges of isolated cat brain after revival from years of frozen storage_ in Brain Research Vol 70, 527-531 (1974)] Michael Price Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=3882