X-Message-Number: 3882
Date: Wed, 22 Feb 1995 11:54:14 GMT
From:  (Michael Clive Price)
Subject: Sternberg article    SCI.CRYONICS

My thanks to Art Quaife for passing on Hal Sternberg's repsonse to my
query about cellular degeneration during storage at approximately -80C. 

> Based on muscle cell contractility after freezing and thawing, 
> and the literature on cell freezing, Hal believes that storage at 
> above -80 degrees C should be for no more than 72 hours.

This seems a rather short period.  How does this square with the
experiments of Suda et al [*] who demonstrated recovery of cortical
functions from storage of up to 7 years at -20C (and perhaps -60C, the
articles are ambiguous on the lower temperature)?

This point of of especial significance to non-US cryonics patients who
get flown to the US on dry-ice.

[*] Isamu Suda, Kyoko Kito and Chizuko Adachi _Viability of Long Term
Frozen Cat Brain in vitro_ in Nature, Vol 212, 268-270 (1966) and
_Bioelectric discharges of isolated cat brain after revival from years
of frozen storage_ in Brain Research Vol 70, 527-531 (1974)]

Michael Price                        

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