X-Message-Number: 3926 Newsgroups: sci.cryonics From: (Michael Clive Price) Subject: Re: -79xC and glycerol Molarity References: <3imv3c$> Date: Sun, 26 Feb 1995 08:51:52 +0000 Message-ID: <> My thanks to Brian Wowk and Mike Darwin for their answers about molarity and cracking. Does anyone remember offhand what the glycerol concentrations were in the patients that Alcor took over control of that showed evidence of cracking? Is it possible that their cracking was due to too low a concentration of CPA? What evidence is there of cracking at high molarities? What I wonder is whether Ettinger's no cracking results (still to be confirmed) are a result of the relativity high CPA concentrations (5-6M) (in comparison with Suda (2M) and Pegg <=3M)) rather than the cooling/re-warming rates. I am delighted to hear that Suda did more work, but why oh why didn't/doesn't he ever publish all the stuff! (Is he still alive, Mike?) BTW I got an email wondering if I really meant that Suda had stored and revived brains from 7 years storage at -20C -- the temperature didn't seem cold enough. Yes, that is what I meant. What Suda's experiments showed was that there was a slow deterioation over this time period (the shorter the storage at -20 C the better the revival, so this isn't evidence in favour of permafrost burials) and the brains stored at lower temperatures (-60C and -90C) were markedly more damaged, presumably (hopefully!) because of the low CPA concentraion used. Michael Price Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=3926