X-Message-Number: 4507 From: Date: Tue, 13 Jun 1995 19:17:08 -0400 Subject: #4503, #4501 About Mike Darwin's #4503: 1. Mike says that blocks of [brain] tissue from the - 90'C studies have in fact been cooled to -196'C and look just as good ultrastructurally. That sounds very important indeed, and ups the ante on separating wheat from chaff (if any) in the BPI/Alcor procedures. 2. Mike intimates, however, that the further-cooled tissue blocks did not yield definitive information regarding cracking at the lower temperatures. He then offers to send us a dog in dry ice for cool-down to LN2 and rewarming, autopsy, and reperfusion. This presumably would allow a determination as to whether the Cryonics Institute's lack of cracking was associated only with cool/warm rate or whether other features of the CI procedure contributed. However, cooling down from dry ice to liquid nitrogen and reverse, by the methods we used with our sheep heads, is so cheap and easy that it doesn't seem sensible to ship dogs here for that purpose--unless, perhaps, space is at a premium at BPI. Also, we do not have the equipment or personnel to do the type of autopsy that is wanted. Nevertheless, if Mike really thinks this would be useful, we are willing to do it, if we can obtain the help of local people (veterinarians?) to do the evaluations. Since this would be a joint investment, we would also have to spell out an agreement with Mike in advance as to just what would be done. We don't want anyone to be disappointed or feel misled. 3. Mike's statement that dog brains are biologically closer to human in some important respects than [other] primate brains is astonishing. I'm not disputing his sources, which I haven't studied--it's just surprising. 4. I'm puzzled by his statement that "dogs and people...both have remarkably well protected heads..." followed by statements that when "when humans stopped swinging from branches and walked upright they took their heads out of the line of fire..." and some other things that don't seem to hang together. But it isn't very important. ------------- About Dave Pizer's #4501: Congratulations on the new research, Alcor and new commercial firm. I wish we could raise $40,000 more for research as easily as Alcor did! (Well, maybe it wasn't easy. Alcor has always earned its luck.) Which reminds me that recently, when I mentioned some people who had given substantial amounts for cryonics research or cryonics generally, I omitted the names of Paul Segall, Hal Sternberg, Harry Waitz et al at BioTime. The reason for the omissions was that I didn't know how much of their work was funded by their own individual, private contributions and was directed to cryonics research as distinct from commercial research directed toward clinical medicine. But maybe that's just a quibble: the above-named do seem to have made significant contributions, judging by brief reports, and we hope these will be spelled out in more detail in coming months. Robert Ettinger Cryonics Institute Immortalist Society Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=4507