X-Message-Number: 5006
Date: Tue, 17 Oct 1995 02:12:34 -0700 (PDT)
From: Doug Skrecky <>
Subject: request for help - second answer

 Suggestion #4: Consider using 2-methoxyethanol. (This is also called
 ethylene glycol monomethyl ether). Tissue permeability of this is
 actually a little better than methanol (20.9 x glycerol). 
 2-Methoxyethanol is completely nontoxic at a 1.3 M concentration and is
 an effective cryoprotectant. (See Cryobiology 398-405 Vol.31 1993 &
 306-312 Vol.30 1993)


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