X-Message-Number: 5006 Date: Tue, 17 Oct 1995 02:12:34 -0700 (PDT) From: Doug Skrecky <> Subject: request for help - second answer Suggestion #4: Consider using 2-methoxyethanol. (This is also called ethylene glycol monomethyl ether). Tissue permeability of this is actually a little better than methanol (20.9 x glycerol). 2-Methoxyethanol is completely nontoxic at a 1.3 M concentration and is an effective cryoprotectant. (See Cryobiology 398-405 Vol.31 1993 & 306-312 Vol.30 1993) Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=5006