X-Message-Number: 7050 Date: Sun, 20 Oct 1996 22:54:45 -0700 (PDT) From: Doug Skrecky <> Subject: Prometheus Project Suggestion Here's a suggestion for the Prometheus research project. Test the efficacy of the following cryopreservation solution in rat hearts. The formula is: Dulbecco's modified Eagle's medium (DMEM), 10 dimethylsulfoxide (DMSO), 20% fetal calf serum (FCS) and 0.1 mol/L trehalose. When this formula was tested it enabled fully reversible cryopreservation of dog trachea rings, which were used as transplants, after storage was in a deep freezer at -85 C for 285 days. Reference: "Reliable Long-Term Cryopreservation of Trachea for Tissue Banks" Transplantation Proceedings Vol.28(2): 1119-1120 1996 Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=7050