X-Message-Number: 7506 Date: Wed, 15 Jan 1997 10:41:44 -0800 From: Tim Freeman <> Subject: Visser agent & toxicity >Moreover, the putative Visser agent, dimethylformamide >(DMF) appears to be highly toxic to kidneys at 40% v/v concentration >(which is well below the minimum 52% concentration needed to vitrify). >While DMF has reduced toxicity at lower concentrations, such as 25%, >the quantities of ice that form with such concentrations are fatal to >kidneys. So claims of perfected kidney preservation appear >particularly implausible. How long does the toxicity take to happen? I thought the idea was to put the cryoprotectant in and later take it out while the organ was cold but still above freezing, before the cryoprotectant kills it. I understand the people who know the answer to this may still be under non-disclosure, so I will understand if there is no answer. Tim frereman Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=7506