X-Message-Number: 7618 From: Jack JWA van Loon <> Newsgroups: sci.cryonics Subject: Freezing cells Date: Sat, 01 Feb 1997 11:26:16 -0800 Message-ID: <> Does any of you have any experience in freezing (and storing) single cells at -20 dgr.C for a prolonged period? I am aware that the best storage temp. is liquid Nitrogen but I only have -20 degr.C for this particular experiment. I have to store the cells for at least a few weeks. What happens if a cell suspension (with DMSO or any other preservative) goes trough a temperature profile of -10 to -70 degr.C? Is there a change in water crystal formation ? Do you know of any references ....? They are very hard to find. Thank you in advance, Jack van Loon Gorinchem, The Netherlands e-mail : Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=7618