X-Message-Number: 7618
From: Jack JWA van Loon <>
Newsgroups: sci.cryonics
Subject: Freezing cells
Date: Sat, 01 Feb 1997 11:26:16 -0800
Message-ID: <>

Does any of you have any experience in freezing (and storing) single
cells at -20 dgr.C for a prolonged period? I am aware that the best
storage temp. is liquid Nitrogen but I only have -20 degr.C for this 
particular experiment. I have to store the cells for at least a few 
What happens if a cell suspension (with DMSO or any other preservative) 
goes trough a temperature profile of -10 to -70 degr.C? Is there a change 
in water crystal formation ?
Do you know of any references ....? They are very hard to find.

Thank you in advance,

Jack van Loon
Gorinchem, The Netherlands
e-mail : 

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