X-Message-Number: 7663
From:  (Thomas Donaldson)
Subject: Re: CryoNet #7655 - #7661
Date: Fri, 7 Feb 1997 10:02:54 -0800 (PST)

Hi again!

To Paul Wakfer:

I feel very glad that your pledge fund has started rising again. We need this
research a great deal --- even if we need other research too, and may in the
end (an end many years from now) find ways to revive even those frozen by
current methods.

To All:

I regret that I could not be present for further examination of the frozen
rat hearts --- clearly it was done much more closely after I left. I will add
that if all else is stripped away, I would still insist that the experiment
be done under conditions in which we can assure ourselves, by thermocouple
measurements rather than guesswork, that the hearts actually were at LN
temperature for 20 minutes or longer. Other things can be measured by other
experiments, but if they cannot be revived (as clearly seems to be true now)
from LN temperatures after 20 minutes, then (unfortunately) Visser's solution
just doesn't work and may be excluded from further consideration.

Certainly, if Visser's procedure isn't well defined, and her cryoprotective
solution isn't well defined, then a lot more work would still need to be done
IF one or more of the simplified experiments had succeeded. I would not even
demand either of these for a first experiment. But I would insist on revival
from 20 minutes at verifiably (by thermocouple, not guesswork) LN temperatures.

			Best and long long life to all,

				Thomas Donaldson

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