X-Message-Number: 7742
Date: Sun, 23 Feb 1997 20:41:01 -0400 (EDT)
Subject: Cryo Journal Club 3

Cryo Journal Club 3

Cryopreservation of Anchorage-Dependent Mammalian Cells Fixed to Structured
Glass and Silicon Substrates


This paper describes a procedure for the cryopreservation of
anchorage-dependent cells in a predefined position on microstructured glass
or silicon substrates. During freezing and thawing, cells retain their
location on the substrate, and an individual comparison and identification
of cells before and after preservation are possible. To utilize this
advantage, a good adherence and a high survival rate are important. It can
be shown that adhesion of mouse fibroblasts (NIH-3T3) to substrate strongly
influences the survival rate: 94% of cells grown for 16 h before freezing
were judged to be alive after thawing. Widely spaced cells are best suited
to cryopreservation on substrates. The different patterns of adhesion of
cells to substrates when incubated for 1, 3, 6, and 16 h, were visualized
by total internal reflection microscopy (TIRM).

Cryobiology, v 33, n 2, April 1996.


One can conclude from this experiment that cells have to be healthy in
order to survive freezing. It is well known that cells that do not adhere
well to culture plates or flasks are unhealthy. The implication for cryonics
is that suspension should be concentrated on a group of cells such as in
the brain in order to maximize revival.

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