X-Message-Number: 7802 Date: Tue, 4 Mar 1997 05:03:18 -0800 (PST) From: Doug Skrecky <> Subject: successful revival of thawed mouse hearts Fetal mouse hearts were revived successfully from liquid nitrogen storage back in 1974 by the MRC Transplantation Group at the University of Alberta in Edmonton, Canada. Cryopreservation fluid included 10% DMSO and 10% FCS (fetal calf serum) in Hepes buffer. Deletion of either of these two components eliminated survival. In all 59% of 54 treated hearts used showed strong electrical activity after being thawed out. Reference: Cryobiology 11: 28-32 1974 Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=7802