X-Message-Number: 8007 Date: Sun, 6 Apr 1997 08:44:20 -0700 (PDT) From: Doug Skrecky <> Subject: 5'th update on fly longevity experiments This is the fifth update on my fly experiments. The day 67 census of the bottles from the first run found a single fly alive in each of the forskolin, NADH and xanthophyll bottles. All flies are dead in the alpha lipoic, biotin, CLA, glutamine, lycopene, pregnenolone, RNA and pyroglutamic bottles. I regard this first run as essentially finished. The preliminary results obtained in the second run were not what I had expected. However I have an explanation for these results, which may even have some human application. Second Run Day 11 Census Group Alive/Dead Survival Control 5/3 63% Nicotinamide 6/1 86 Coenzyme Q10 7/0 100 Nicotinamide/CoQ10 6/1 86 Acetylcarnitine 6/1 86 ALC/COQ10 7/0 100 Basil 4/1 80 Bromelain 7/2 78 Caraway 4/2 67 Chlorophyll 7/0 100 Cloves 5/1 83 Cumin 7/0 100 Curcumin 5/2 71 Dextromethorphan 5/1 83 Fenugreek 4/1 80 Ginger 4/2 67 Green Tea 6/0 100 Leucoanthocyanins 6/1 86 Mace 5/2 71 Nutmeg 5/2 71 Oregano 7/1 88 Rosemary 9/1 90 Sage 8/0 100 Thyme 8/0 100 Some spices with bactericidal properties maintained survival at 100%. Although there has been much talk about free radicals and aging, in the present case my guess is that it is pathogens that are proving to be the main longevity limiting factor. Although drosophilia medium 4-24 does contain a mold inhibitor, I suspect that it does not offer adequate protection against bacteria and viruses. When I examined the scientific literature on the life spans of control Oregon R drosophilia melanogaster flies fed medium 4-24 I found great variations. Although some experiments found average life spans of around 55 days at room temperature, one found this to be as high as 81 days. Why the variation? This same type of inconsistancy occurs with rodents as well. For example in one experiment the average life span of control Wistar rats was found to be 75.5 weeks, while rats that were calorie restricted lived 138.2 weeks. (Gerontology 28: 233-241 1982) This is strong support for the effectiveness of caloric restriction in prolonging life span. However in another experiment control Wistar rats lived to an average of 133.1 weeks, while rats that were calorie restricted after 52 weeks of age lived an average of 150 weeks. (Journal of Gerontology 41(1): 13-19 1986) Control rats live either 75.5 or 133.1 weeks for apparently no reason at all if pathogens are not a factor. Pathogens may have a significant effect on human longevity. Viruses can cause cancer, while some bacteria are suspected of promoting cardiovascular disease. Pathogens may be an primary, though indirect cause of age associated mortality in humans, as well as in rats and flies. One of the active ingrediants in Listerine mouthwash is thymol, which is derived from thyme. I am starting some new breeding bottles which contain added thyme to act as a bactericide. Flies that are born in these I will use to start my third run, which will also contain a bactericide (possibly thyme) in all of the bottles to give a level playing field. If pathogens are killing off flies prematurely I want to eliminate this factor before testing other things. Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=8007