X-Message-Number: 8280 Date: Wed, 4 Jun 1997 10:34:09 -0400 (EDT) From: "Kevin Q. Brown" <> Subject: Cryopreservation of Mouse Embryos Appended below is a query from Juan Carlos Silva, who plans to cryopreserve mouse embryos at CalTech and has some questions about the procedure. (I offered to forward his query to CryoNet.) Any assistance that the CryoNet readers can provide would be appreciated. Thanks. Kevin Q. Brown PS: His email address is "". -- > Date: Mon, 02 Jun 1997 15:41:25 -0800 > From: Juan Carlos Silva <> > Organization: California Institute of Technology > To: > Subject: Cryopreservation of Mouse Embryos. Mr Kevin Q. Brown, I'm currently working for the California Institute of Technology with transgenic mice. Very soon I'll start freezing mouse embryos and I have already the equipment, but there are a few details that I would like to check first with somebody with experience. Do you know where I should ask??? Regards Juan Silva [ A followup message provided more details about his questions. - KQB ] -Do you freeze embryos in straws or in vials? -If straws, what supplier you use and diameter of straw. -How do you identify the straws?? -Do you freeze the embryos in ethanol bath or in a chamber with liquid nitrogen vapor. -Do you have any problem in transfering the embryos from the chamber to the tank? -How do they storage in liquid nitrogen. -What make and model of tank for storing frozen embryos you use. You can post my questions as you mention before and include my e-mail address. Thank you again for your collaboration. Juan Silva -- Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=8280