X-Message-Number: 8597 Date: Mon, 15 Sep 1997 18:49:38 -0700 (PDT) From: Doug Skrecky <> Subject: 16'th update on fly longevity experiments This is the sixteenth update of my fly experiments. The heat wave is over so I expect life spans will be greater in the sixth run than in the fifth run. In the fifth run all that looks interesting is activated charcoal, sucrose and l-proline. Since activated charcoal significantly outperformed controls in the 3'rd run, I have shortlisted this as a "probable" life span prolonging supplement for flies. Since the lower dosage of a supplement almost always results in a greater life span, I am decreasing the dosages I use in the sixth run. I am retesting l-proline as well as sage and paprika here. For the first time I am boiling the water I use. Although flies live longer when live yeast is added to their food, this result was obtained under highly sterile laboratory conditions. This might not apply to my less than completely sanitary home experiments. So I am running two control bottles, one with added yeast from each breeding bottle to test for the effect of live yeast. None of the supplement bottles contains live yeast in this run. The breeding bottles were all created 20 days ago, using boiled water and a high dose (1.04 gm) of activated charcoal added to 20 gm 4-24 medium. Fifth Run Survival BB# Supplement DAY 16 DAY 23 DAY 26 DAY 30 DAY 36 1 control#1 10% 10% 0% 0% all 0% 1 l-alanine 13 0 0 0 1 l-alanine 4X 0 0 0 0 1 anise 10 0 0 0 1 anise 4X 0 0 0 0 1 l-glutamine 13 0 0 0 1 l-glutamine 4X 0 0 0 0 1 malt 25 0 0 0 1 malt 4X 9 0 0 0 1 paba 15 0 0 0 1 paba 4X 0 0 0 0 2 control#2 22 13 4 0 2 l-cysteine 18 0 0 0 2 l-cysteine 4X 0 0 0 0 2 l-glycine 10 0 0 0 2 l-glycine 4X 0 0 0 0 2 l-isoleucine 11 0 0 0 2 l-isoleucine 4X 3 0 0 0 2 l-leucine 11 0 0 0 2 dl-phenylalanine 8 0 0 0 2 dl-phenylalanine 4X 17 0 0 0 2 l-proline 27 13 7 7 2 l-proline 4X 26 5 0 0 2 l-threonine 22 6 6 0 2 l-threonine 4X 0 0 0 0 2 l-valine 13 4 0 0 2 l-valine 4X 17 0 0 0 3 activated charcoal 27 13 0 0 3 activated charcoal 4X 40 20 20 0 3 glucose 24 8 8 0 3 glucose 4X 28 9 0 0 3 l-leucine 4X 6 0 0 0 3 sorbitol 25 8 4 0 3 sorbitol 4X 19 6 0 0 3 sucrose 32 21 11 0 3 sucrose 4X 15 0 0 0 4 control#4 65 44 20 8 4 l-carnosine 39 25 13 4 4 l-carnosine 2X 55 27 12 0 4 l-carnosine 4X 33 7 3 3 4 l-carnosine 8X 15 3 3 0 Sixth Run BB# Supplement Amount 1 cntl 1 1 cntl 1 +yeast 1 B complex: 1/4 capsule 1 B complex 4X: 1 capsule: (50 mg B1, 50 mg B2, 50 mg B3, 50 mg B5, 50 mg B6, 50 mcg B12, 60 mcg biotin, 1 mg folic acid, 50 mg choline bitartrate, 50 mg inositol, 50 mg PABA) 1 chitosan: 1/4 capsule 75 mg 1 chitosan 4X: 1 capsule 300 mg 1 chondroitin sulfate: 1/4 capsule 100 mg 1 chondroitin sulfate 4X: 1 capsule 400 mg 1 chromium picolinate: 1/4 capsule 125 mcg 1 chromium picolinate 4X: 1 capsule 500 mcg 1 cinnamon: 1/16 teaspoon 1 cinnamon 4X: 1/4 teaspoon 1 DMAE bitartrate: 1/4 capsule 37.5 mg 1 DMAE bitartrate 4X: 1 capsule 150 mg 2 cntl 2 2 cntl 2 +yeast 2 french mushroom: 1/16 teaspoon 2 french mushroom 4X: 1/4 teaspoon 2 kava kava: 1/4 capsule 11.25 mg kavalactones 2 kava kava 4X: 1 capsule 45 mg kavalactones 2 onion: 1/16 teaspoon 2 onion 4X: 1/4 teaspoon 2 paprika, spanish: 1/16 teaspoon 2 paprika, spanish 4X: 1/4 teaspoon 2 l-proline: 1/4 capsule 125 mg 2 l-proline 4X: 1 capsule 500 mg 2 sage: 1/16 teaspoon 2 sage 4X: 1/4 teaspoon 2 sodium citrate: 1/16 teaspoon 375 mg 2 sodium citrate 4X: 1/4 teaspoon 1500 mg 3 cntl 3 3 cntl 3 +yeast 3 St John's Wort: 1/4 capsule 225 mcg hypericin 3 St John's Wort 4X: 1 capsule 900 mcg hypericin 3 sumac: 1/16 teaspoon 3 sumac 4X: 1/4 teaspoon 3 wild yam: 1/4 capsule 125 mg 3 wild yam 4X: 1 capsule 500 mg Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=8597