X-Message-Number: 8700
Date: Tue, 28 Oct 1997 08:35:42 +0000
From: randy <>
Subject: Re: CryoNet #8699

At 05:00 AM 10/28/97 -0500, you wrote:
>CryoNet - Tue 28 Oct 1997
>
>    #8699: Re: CryoNet #8697 - #8698 [Kennita Watson]
>
>----------------------------------------------------------------------
>
>Message #8699
>Date: Mon, 27 Oct 1997 08:27:07 +0000
>From: Kennita Watson <>
>Subject: Re: CryoNet #8697 - #8698
>References: <>
>
>> From:  (Randy)
>> Subject: In-Situ freezing of Rabbit and Rat Brain
>> Newsgroups: sci.cryonics
>>
>> There is apparently an article in the Sept 97 issue of Cryobiology
>> magazine that may be of some interest to cryonicists. From their
>> website I copied the following:
>>
>> M. W. Roos, A. Johansson, and G. O. Sperber. In Situ Freezing of the
>> Rabbit and Rat Brain..........187
>>
>> Has anyone read this?
>> Randy
>> Cryonics: Gateway to the Future?
>> http://members.wbs.net/homepages/c/r/y/cryofan1.html
>> ***********************************
>>
>I saw no reference to rabbits or rats on your Web site, which was
>the only URL included in your message.  Please cut and paste the URL
>along with the title of the article.  I can't double-click a title
>from my mailer to get to the appropriate page -- maybe in Version 5,
>Netscape Mindreader :-) ...

Sorry, didn't include the URL, but here is one with table of contents of
Sept 97:
http://sapphire.surgery.wisc.edu/cryobiology/journal/vol35no2.html

Here's an abstract of the article (also available on their website if you
mess around enough):
>>In Situ Freezing of the Rabbit and Rat Brain
M. W. Roos, A. Johansson, G. O. Sperber


>>We have measured the effectiveness of different standard cryogenic
liquids (liquid nitrogen, chilled isopentane, chilled hexane, and
chilled ethanol) and applied a mathematical model for calculating the
temperature changes at different depths in rabbit and rat heads cooled
in liquid nitrogen. We have also evaluated the extent to which the
freezing time is shortened when the skin is removed prior to immersion.
We conclude that: (i) of the coolants used, liquid nitrogen gives the
most rapid freezing. (ii) dry ice-chilled ethanol is as effective as dry
ice-chilled hexane. Liquid nitrogen and ethanol are safer for the
environment than isopentane and hexane and should satisfy most needs.
(iii) By removing the skin prior to immersion, the freezing time can be
reduced by about 20 s for the rabbit cortex and rat hypothalamus, 30 s
for the rat cortex, and 40 s for the rabbit hypothalamus.

Cryobiology, v 35, n 2, September 1997, p187-191 (ID CY972032)
Copyright =A9 1997 Academic Press
>>

Doesn't look like there was much to their experiment?

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