X-Message-Number: 9344
Date: Tue, 24 Mar 1998 08:58:17 -0800 (PST)
From: Doug Skrecky <>
Subject: storage using DMSO at -74 C is damaging 

Authors
  Allyson K.  Abrams-Ogg AC.  Johnstone IB.
Institution
  Department of Clinical Studies, Ontario Veterinary College, University of
  Guelph, Canada.
Title
  Room temperature storage and cryopreservation of canine
  platelet concentrates.
Source
  American Journal of Veterinary Research.  58(11):1338-47, 1997 Nov.
Abstract
  OBJECTIVE: To determine whether in vitro viability and function, and
  microbiological sterility, of canine platelet concentrates (PC) could be
  retained during storage at 20 to 24 C (room temperature [RT]) for up to 7
  days and cryopreservation for 6 months. ANIMALS: 14 mature
  dogs. PROCEDURE: PC prepared by centrifugation of fresh blood were stored for
  7 days at RT with continuous agitation. An aliquot of each was cryopreserved
  with 6% dimethyl sulfoxide at -74 C for 6 months. Fresh PC (day 0) were
  tested by microbial culture and measurement of platelet count, mean platelet
  volume, pH, glucose and lactate concentrations, lactate dehydrogenase
  activity, response to hypotonic stress, and aggregation. Tests were also
  performed on PC stored at RT on days 3, 5, and 7, and on the cryopreserved
  aliquots after thawing. RESULTS: After 7 days at RT, microbial growth was not
  evident, and decrease in platelet number was not significant. On the basis of
  pH and glucose and lactate concentrations, metabolic activity was maintained
  throughout RT storage. On the basis of mean platelet volume and lactate
  dehydrogenase activity, platelet swelling and membrane damage had occurred.
  Aggregatory responses decreased during RT storage, and platelets recovered
  poorly from hypotonic stress. After cryopreservation for 6
  months, microbial growth was not evident, but platelet numbers were
  significantly decreased. Mean platelet volume and lactate dehydrogenase
  activity were significantly greater, compared with values for day-0 PC.
  Crypreserved platelets aggregated poorly and did not respond to hypotonic
  stress. CONCLUSIONS: Platelet viability and microbiological sterility are
  retained in canine PC stored for 7 days at RT, but platelet function
  pregressively decreases and day-7 platelets are substantially damaged.
  Crypreservation of PC results in considerable damage, compared with that of
  PC stored at RT. CLINICAL RELEVANCE: Similar to human PC, canine PC stored at
  RT for up to 5 days can be recommended for treatment.

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