X-Message-Number: 9392 Date: Thu, 02 Apr 1998 12:36:58 -0800 From: Paul Wakfer <> Newsgroups: sci.cryonics Subject: Hippocampal Slice Cryopreservation Project (Budget) On both the Cryonet and sci.cryonics postings the tabular budget of this project was so messed up as to be virtually undecipherable. So here it is again reformatted and hopefully readable. Please seriously consider funding an item on the equipment list. Your life may be saved as a result! Budget Salary: Dr. Yuri Pichugin, Research Associate 40,000 Cost of Fringe Benefits 12,800 Supplies: Videotapes 150 Anaesthetics, cryoprotectants 3,500 Perfusates and artificial cerebrospinal fluid 8,000 Liquid Nitrogen and carbogen 400 Suture, cannula tubing, rat disposal bags 250 Glassware, plasticware (fabrication of 8 Scot chambers) 2,000 RH-414 fluorescent dye 250 Animal Purchases: 130 rats @ $10.50/rat 1,365 Animal Services: 14 days/rats @ $0.70/day 1,274 One publication with color figures, page reprint charges 3,200 Travel for training plus one presentation 1,500 ------- Total Cost to be paid by RI $72,050 Equipment: Software: Axon Imaging Workbench 2.0, Sigma Plot, SigmaStat 4,500 Image recording system: MotionScope plus frame grabber PCI 2000 27,000 Zeiss microscope, epifluorescent capability 23,000 Brain slice chambers (2), Fine Science, 2,840 Superfusion equipment, sterilizing filters plus roller pump 2,000 Micromanipulator, for nanodrop positioning, Fine Science MM3 1,085 Cryo-Med controlled rate freezer 15,000 Pentium II PC, 300 MHz, 64 MB RAM, 4GB Disk, Zip Drive, CD 3,000 ------- Total Cost of Equipment supplied by INC $73,925 ======Total Project Cost $145,975 -- Paul -- Voice/Fax: 909-481-9620 Page: 800-805-2870 The Institute for Neural Cryobiology - http://www.neurocryo.org Perfected cryopreservation of Central Nervous System tissue for neuroscience research and medical repair of brain diseases Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=9392