X-Message-Number: 9392
Date: Thu, 02 Apr 1998 12:36:58 -0800
From: Paul Wakfer <>
Newsgroups: sci.cryonics
Subject: Hippocampal Slice Cryopreservation Project (Budget)

On both the Cryonet and sci.cryonics postings the tabular budget of this
project was so messed up as to be virtually undecipherable. So here it
is again reformatted and hopefully readable.

Please seriously consider funding an item on the equipment list.
Your life may be saved as a result!

Budget

Salary:  

 Dr. Yuri Pichugin, Research Associate               40,000
               Cost of Fringe Benefits               12,800
Supplies: 

Videotapes                                              150
Anaesthetics, cryoprotectants                         3,500
Perfusates and artificial cerebrospinal fluid         8,000
Liquid Nitrogen and carbogen                            400
Suture, cannula tubing, rat disposal bags               250
Glassware, plasticware 
	(fabrication of 8 Scot chambers)              2,000
RH-414 fluorescent dye                                  250
Animal Purchases: 130 rats @ $10.50/rat               1,365
Animal Services: 14 days/rats @ $0.70/day             1,274
One publication with color figures, page
	reprint charges                               3,200
Travel for training plus one presentation             1,500
                                                    -------
Total Cost to be paid by RI                         $72,050

Equipment: 

Software: Axon Imaging Workbench 2.0,
	 Sigma Plot, SigmaStat                       4,500
Image recording system: MotionScope
	plus frame grabber PCI 2000                 27,000
Zeiss microscope, epifluorescent capability         23,000
Brain slice chambers (2), Fine Science,              2,840
Superfusion equipment, sterilizing filters
	plus roller pump                             2,000
Micromanipulator, for nanodrop positioning,
	Fine Science MM3                             1,085
Cryo-Med controlled rate freezer                    15,000
Pentium II PC, 300 MHz, 64 MB RAM,
	4GB Disk, Zip Drive, CD                      3,000
                                                   -------
Total Cost of Equipment supplied by INC            $73,925

                                                   ======Total Project Cost
                                                   
                                                   $145,975


-- Paul --

 Voice/Fax: 909-481-9620 Page: 800-805-2870
The Institute for Neural Cryobiology - http://www.neurocryo.org
Perfected cryopreservation of Central Nervous System tissue
for neuroscience research and medical repair of brain diseases

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