X-Message-Number: 9548
Date: Sat, 25 Apr 1998 21:58:14 -0700 (PDT)
From: Doug Skrecky <>
Subject: ethylene glycol, trehalose and polyvinylpyrrolidone

Authors
  Saha S.  Otoi T.  Takagi M.  Boediono A.  Sumantri C.  Suzuki T.
Institution
  United Graduate School of Veterinary Science, Yamaguchi University, Japan.
Title
  Normal calves obtained after direct transfer of vitrified bovine embryos
  using ethylene glycol, trehalose, and polyvinylpyrrolidone.
Source
  Cryobiology.  33(3):291-9, 1996 Jun.
Abstract
  In the present study, IVF bovine embryos were vitrified using as the
  cryoprotectants, ethylene glycol plus trehalose plus the polymer,
  polyvinylpyrrolidone (PVP). In Experiment I, toxicity of the vitrification
  solution (VS) containing 20% PVP was tested in relation to temperature and
  exposure time. One hundred percent embryo development was observed with
  treatment at 5 degrees C for 5 min, whereas only 55.5% embryos were developed
  when the treatment was carried out at 20 degrees C for 5 min. In Experiment
  II, embryos were vitrified using one of the three treatments (Treatment A,
  40% ethylene glycol (EG); treatment B, 40% EG + 11.3% trehalose; and
  treatment C, 40% EG + 11.3% trehalose + 20% PVP and rehydrations) was
  performed directly in mPBS. Highest development (84.1%) and hatching rate
  (68.2%) were obtained when embryos were vitrified with the vitrification
  solution used in treatment C. In Experiment III, embryos were vitrified as in
  Experiment II (treatment C). The development and hatching rates were compared
  after rehydration in different rehydration solutions. No significant
  difference was observed among the development and hatching rates when
  rehydration was carried out in different concentrations of trehalose. Five
  vitrified-warmed bovine embryos were transferred directly to five recipients
  and three recipients gave birth to three normal calves.

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