X-Message-Number: 9746 Date: Tue, 19 May 1998 20:57:31 -0700 (PDT) From: Doug Skrecky <> Subject: 21'st update on fly longevity experiment This is the 21'st update on my fly longevity experiments. Since flies used in run #8 had been pre-aged in holding bottles containing taurine larvicide for 29 days prior to use, the remaining life spans are shorter than those from the previous run. The results were very disappointing with acetyl-carnosine, and I have no explanation for why the flies given this died so quickly. However the poor result with almond powder was anticipated. This was the first time I have tested something that I was fairly confident would reduce life span. Unlike mammals, the dietary "essential" fatty acid requirement for flies is zero, and all tests with various added fats have yielded reduced life spans. However monosaturated fats had never been tested before, so I decided to try this with the almond powder. It looks like aged garlic powder, and the lower dosage of chili have a mild pro-longevity effect. Both the lower doses of cardamon, and corriander might also be slightly beneficial before tissue levels accumulate to a toxic level. I will have to check out extra low concentrations of these some time. For the ninth run I am testing a very high dosage (8X) of hawthorn, since the Hawthorn 4X did so well in the seventh run. Otherwise I am continuing to test the spice cabinet in this latest run. Flies were pre-aged for only 17 days, since about 75% of them had died by this time. This shorter holding period may be due to the current higher temperatures accelerating age associated mortality in drosophilia. These insects now show great reluctance in attempting flight, which is typical of aged flies. Eighth Run #NUMBER PERCENTAGE SURVIVAL ON DAY Supplement OF FLIES 3 8 11 14 19 22 26 29 34 39 ___________________________________________________________ cntl 1 11 64 36 27 18 9 9 9 9 0 - cntl 2 9 67 44 33 33 22 22 11 0 - - acetyl-carnosine 7 14 14 0 - - - - - - - acetyl-carnosine 4X 8 75 13 13 0 - - - - - - aged garlic 14 86 50 43 43 21 7 7 7 7 0 aged garlic 4X 10 90 50 50 40 30 10 10 0 - - almond 7 43 14 0 - - - - - - - cardamon 9 78 56 44 44 44 33 22 22 0 - cardamon 4X 10 80 40 20 10 0 - - - - - chili 9 67 56 33 44 22 11 11 11 11 0 chili 4X 7 86 57 43 29 29 14 0 - - - coriander 9 100 78 67 33 33 22 11 0 - - coriander 4X 6 67 50 50 17 0 - - - - - echinacea 9 78 11 11 0 - - - - - - echinacea 4X 13 85 31 31 23 15 0 - - - - tomato 10 50 10 10 10 10 10 0 - - - tomato 4X 14 93 29 21 21 7 0 - - - - water reduced 9 67 33 33 33 33 0 - - - - NINETH Run Cntl 1 Cntl 2 Horseradish: 1/16 tsp Horseradish 4X: 1/4 tsp Hawthorn 4X: 500 mg Hawthorn 8X: 1000 mg Myrrh: 1/16 tsp Myrrh 4X: 1/4 tsp Orange peel: 1/16 tsp Orange peel 4X: 1/4 tsp Orris root: 1/16 tsp Orris root 4X: 1/4 tsp Pepper (white): 1/16 tsp Pepper (white) 4X: 1/4 tsp Peppermint leaves: 1/16 tsp Peppermint leaves 4X: 1/4 tsp Saffron: 1/16 tsp Saffron 4X: 1/4 tsp Sasparilla: 1/16 tsp Sasparilla 4X: 1/4 tsp Savory: 1/16 tsp Savory 4X: 1/4 tsp Senna: 1/16 tsp Senna 4X: 1/4 tsp Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=9746