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Msg  Description
# 14309 Re: CryoNet #14282 - Crippling Vitrification (Installment #1) [Paul Wakfer]
  is the first installment of my reply. After writing the above and being almost ready . . . same "fundraiser" tactics again! As I have stated several times before, I took that action . . . postings ('99%' of which are 'busy with brain > masturbation'); Whether intentionally or unintentionally Pascal has . . . with their own, or each other's brains. > of cryonicists ('short-sighted, head-in-the- . . . they really don't want life-extension after all. > and, lastly, 'the intransigent, pig-headedness > . . . well summarized my post. However as I stated above in reply to Pascal's quote . . . else. What do you learn about > the state of vitrification from it? We already know . . . to a site that summarizes the >> current state-of-the-art. My text quoted below . . . itself induced additional unrecoverable damage, > and soon after abandoned the pressure approach. This is a . . . of this particular informative answer is not stated. > Has 21CM in fact done any actual brain research at all? This question of Pascal' . . . answer. > Wakfer: 21CM is not working with > brain slices. That is the research project of . . . improved vitrification fluids that >> by themselves leave brain slices ~95 percent functional (not after >> freeze-and-thaw, just after exposure to the fluid.) and the full . . . fall downstairs and lie there > for several hours or days before being reached by a
(Mon, 14 Aug 2000, 39 KB)
# 0019.3 The Technical Feasibility of Cryonics; Part #3 [Ralph Merkle]
  theoretic death has not occurred, a frozen brain is not a healthy structure. While repair . . . frozen tissue to a healthy and functional state ever prove feasible? If the answer is " . . . sufficient to permit inference of the healthy state with memory and personality intact, then repair . . . restore it to a healthy and functional state. 3.) Take the original molecules and move . . . is easier to evaluate. Overview of the Brain. The brain has a volume of 1350 cubic centimeters ( . . . about three pounds). The smallest normal human brain weighed 1100 grams, while the largest weighed . . . material[16, page 419]. Thus, an average brain has slightly over 100 grams of protein, . . . is to keep the tissue frozen until after all the desired structural information is recovered. . . . can be taken to restore the structure after it has been analyzed. Repair or Replace? . . . must analyze the structure to determine its state. The primary purpose of this phase is . . . used if analysis were to take place after warming. We will not explore these possibilities . . . board repair is determination of the healthy state. In this phase, the structural information derived . . . power costs about 10 cents per kilowatt hour. Future costs of power will almost certainly
(22 Nov 92 21:15:56, 56 KB)
# 5772 Trans Time Newsletter [quaife]
  below 20 degrees C for nearly one hour. Dogs (2) and baboons (3) have survived blood-substituted hypothermia for 1-3 hours. Hamsters (4) have recovered from partial-freezing . . . in nature, frogs may overwinter in these states (5). While some invertebrates can tolerate freezing . . . venous effluent removed. Their hearts were stopped after 30-40 mEQ/ml KCl was added . . . 10 degrees C for 4-4.5 hours. Minimum esophageal temperatures ranged from 0.8- . . . evoked potentials, intracranial and central arterial pressures, brain, esophageal and rectal temperatures, placed on cardio-
(Tue, 20 Feb 1996, 15 KB)
# 13244 Re: CryoNet #13228: Belated Remarks on Cryonics and Abortion [Paul Wakfer]
  in > vitro fertilization) clinics throughout the Unites States and elsewhere. > The reason is fertility drugs. . . . if many of the are not viable after thawing it does not matter. Only one . . . the optimal period being within 18.0 hours after > conception, or so I ve read. The . . . child s living > infant body, complete with brain, heart, lungs, fingers, toes, organs, > fingerprints, nervous
(Fri, 11 Feb 2000, 8 KB)
# 13754 please unregister me out of the maling list [Pierre Le Bert]
  that the electrons were in the ground state > for the specific positions of the nucleii, . . . the electrons to > be in the ground state almost always (barring things like spin > orientation in free radicals) gives a unique electronic state. > Now electronically excited states _do_ have different electron > distributions and different potential energy surfaces, and they > are important during transition states of reactions, but I doubt > that a . . . 3 in 5% CO2. Neurons die within hours > in this or in other 26 mM . . . can also be used to store viable brain tissue for up to a > month with . . . Rat embryo hippocampi have 100% cell viability after > 1 week of storage in Hibernate/B27.
(Sat, 20 May 2000, 8 KB)
# 13228 Belated Remarks on Cryonics and Abortion [David Pascal]
  in vitro fertilization) clinics throughout the Unites States and elsewhere. The reason is fertility drugs. . . . the optimal period being within 18.0 hours after conception, or so I ve read. The . . . child s living infant body, complete with brain, heart, lungs, fingers, toes, organs, fingerprints, nervous
(Tue, 8 Feb 2000, 21 KB)
# 4616 Critique of CI Sheep Brain Results [Mike Darwin]
  compuserve.com> Subject: Critique of CI Sheep Brain Results CRITIQUE AND COMMENTS ON "The Effect of Freeze-Thawing on the Structure of Glycerolized Brains of the Sheep by Mike Darwin Introduction: . . . a glycerolized, and a glycerolized frozen- thawed brain (i.e., two animals at a minimum). . . . anesthetized and cannulated in a beating heart state? Were these heads collected postmortem (i.e., after slaughter)? If the heads were removed after slaughter how was introduction of air into . . . how were they killed? In the United States and most Western countries agricultural animals which . . . voltage/amperage alternating current through the head (brain). The animals are then hoisted into the . . . in serious pre-cryopreservation injury to the brain and would be a serious source of . . . all domestic animals slaughtered in the United States and Europe undergo this kind of stunning . . . tissue "takes up" the stain or appears after staining speaks not only to the visible . . . sections cut from the brain for fixation after cryopreservation? Was the fixative warm or cold . . . minutes of warm ischemia followed by 24 hours on ice (with blood present). My *impression*
(06 Jul 95 02:55:52, 19 KB)
# 10355 Re: CryoNet #10344 Suspension Damage [Paul Wakfer]
  the effect that the molecules in our brain are > broken down to smaller ones. Given . . . room temperature this requires at least 12 > hours. As you know, cryonicists attempt to get . . . loss of order, reduction to lowest energy states, diffusion of solutes to equiconcentration throughout, etc.) . . . experiments that such decay is fully reversible after 17 minutues of normothermic ischemia and after
(Sun, 30 Aug 1998, 8 KB)
# 7454 Suicide and cryonics [Michael Darwin]
  not somebody who decided to do this after they got sick, >society -- even I -- would . . . proposition as has been so often been stated by its promoters (including me, in the . . . dying -- all to provide protection for their brains in the hope of return to life . . . find a glioblastoma eating away at your brain and by the time your quality of . . . you won't have much of a brain left either. Many people without primary brain disease nevertheless find themselves with metastases of cancer to their brain which present terrible choices. I watched Jim . . . cards with friends and then a few hours later be perfusing him. These are, however,
(08 Jan 97 07:13:28, 8 KB)
# 24245 Oreg'n, Asst'd Suic'de & Hypoth'rmia [RAMole]
  terminal agonies and deterioration of body and brain, and to die at a known time . . . promptly, and to die of hypothermia so brain deterioration is delayed for 45 minutes or even several hours, Oregon's Assisted Suicide Law may provide . . . coldness of the water. Within a half hour the water would be cold, and within an hour or two he'd die of hypothermia, . . . and plenty of time to work before brain deterioration began. Details: Too many cryo patients die, then lie around for hours before they are discovered to be dead, . . . they die at normal body temperature, the brain deteriorates beyond recovery by conventional medical technology . . . everyone present, and to die cold so brain deterioration is delayed for a long time. . . . out and submitted. (Maybe not submitted until after death; this part is not clear to . . . minutes. And dies in one to three hours, usually. The above fills all requirements except . . . actual Oregon statute at http://www.leg.state.or.us/ors/127.html Here is
(Mon, 14 Jun 2004, 24 KB)

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