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Msg | Description |
# 14309 | Re: CryoNet #14282 - Crippling Vitrification (Installment #1) [Paul Wakfer] |
is the first installment of my reply.
After writing the above and being almost ready . . . same "fundraiser" tactics again!
As I have stated several times before, I took that action . . . postings ('99%' of which are 'busy with brain
> masturbation');
Whether intentionally or unintentionally Pascal has . . . with their
own, or each other's brains.
> of cryonicists ('short-sighted, head-in-the- . . . they really don't want life-extension after all.
> and, lastly, 'the intransigent, pig-headedness
> . . . well summarized my post.
However as I stated above in reply to Pascal's quote . . . else. What do you learn about
> the state of vitrification from it?
We already know . . . to a site that summarizes the
>> current state-of-the-art.
My text quoted below . . . itself induced additional unrecoverable damage,
> and soon after abandoned the pressure approach.
This is a . . . of this particular informative answer is not
stated.
> Has 21CM in fact done any actual brain research at all?
This question of Pascal' . . . answer.
> Wakfer: 21CM is not working with
> brain slices. That is the research project of . . . improved vitrification fluids that
>> by themselves leave brain slices ~95 percent functional (not after
>> freeze-and-thaw, just after exposure to the fluid.)
and the full . . . fall downstairs and lie there
> for several hours or days before being reached by a (Mon, 14 Aug 2000, 39 KB) |
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# 0019.3 | The Technical Feasibility of Cryonics; Part #3 [Ralph Merkle] |
theoretic death has not occurred, a frozen brain is
not a healthy structure. While repair . . . frozen tissue to a healthy and functional
state ever prove feasible? If the answer is " . . . sufficient to permit inference of the healthy state with
memory and personality intact, then repair . . . restore it to a healthy and functional state.
3.) Take the original molecules and move . . . is easier to evaluate.
Overview of the Brain.
The brain has a volume of 1350 cubic centimeters ( . . . about three
pounds). The smallest normal human brain weighed 1100 grams, while the
largest weighed . . . material[16, page 419].
Thus, an average brain has slightly over 100 grams of protein, . . . is to keep the tissue frozen until after all the desired
structural information is recovered. . . . can be taken to restore
the structure after it has been analyzed.
Repair or Replace?
. . . must analyze the structure to determine its
state. The primary purpose of this phase is . . . used if analysis were to take place after warming. We will not explore
these possibilities . . . board repair is determination of the healthy
state. In this phase, the structural information derived . . . power costs about 10 cents per kilowatt hour. Future costs of
power will almost certainly (22 Nov 92 21:15:56, 56 KB) |
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# 5772 | Trans Time Newsletter [quaife] |
below 20 degrees C for nearly one hour. Dogs (2) and baboons (3)
have survived blood-substituted hypothermia for 1-3 hours.
Hamsters (4) have recovered from partial-freezing . . . in
nature, frogs may overwinter in these states (5). While some
invertebrates can tolerate freezing . . . venous effluent removed.
Their hearts were stopped after 30-40 mEQ/ml KCl was added . . . 10 degrees C
for 4-4.5 hours. Minimum esophageal temperatures ranged from 0.8-
. . . evoked potentials, intracranial and
central arterial pressures, brain, esophageal and rectal
temperatures, placed on cardio- (Tue, 20 Feb 1996, 15 KB) |
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# 13244 | Re: CryoNet #13228: Belated Remarks on Cryonics and Abortion [Paul Wakfer] |
in
> vitro fertilization) clinics throughout the Unites States and elsewhere.
> The reason is fertility drugs. . . . if many of the are not
viable after thawing it does not matter. Only one . . . the optimal period being within 18.0 hours after
> conception, or so I ve read. The . . . child s living
> infant body, complete with brain, heart, lungs, fingers, toes, organs,
> fingerprints, nervous (Fri, 11 Feb 2000, 8 KB) |
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# 13754 | please unregister me out of the maling list [Pierre Le Bert] |
that the electrons were in the ground state
> for the specific positions of the nucleii, . . . the electrons to
> be in the ground state almost always (barring things like spin
> orientation in free radicals) gives a unique electronic state.
> Now electronically excited states _do_ have different electron
> distributions and different potential energy surfaces, and they
> are important during transition states of reactions, but I doubt
> that a . . . 3 in 5% CO2. Neurons die within hours
> in this or in other 26 mM . . . can also be used to store viable brain tissue for up to a
> month with . . . Rat embryo hippocampi have 100% cell viability after
> 1 week of storage in Hibernate/B27. (Sat, 20 May 2000, 8 KB) |
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# 13228 | Belated Remarks on Cryonics and Abortion [David Pascal] |
in
vitro fertilization) clinics throughout the Unites States and elsewhere.
The reason is fertility drugs. . . . the optimal period being within 18.0 hours after
conception, or so I ve read. The . . . child s living
infant body, complete with brain, heart, lungs, fingers, toes, organs,
fingerprints, nervous (Tue, 8 Feb 2000, 21 KB) |
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# 4616 | Critique of CI Sheep Brain Results [Mike Darwin] |
compuserve.com>
Subject: Critique of CI Sheep Brain Results
CRITIQUE AND COMMENTS ON "The Effect of Freeze-Thawing on
the Structure of Glycerolized Brains of the Sheep
by Mike Darwin
Introduction: . . . a glycerolized, and a glycerolized frozen-
thawed brain (i.e., two animals at a minimum).
. . . anesthetized and cannulated in a beating heart state?
Were these heads collected postmortem (i.e., after
slaughter)? If the heads were removed after slaughter how
was introduction of air into . . . how were they killed? In the
United States and most Western countries agricultural
animals which . . . voltage/amperage alternating current through
the head (brain). The animals are then hoisted into the
. . . in serious pre-cryopreservation injury to the brain and
would be a serious source of . . . all
domestic animals slaughtered in the United States and Europe
undergo this kind of stunning . . . tissue "takes up" the stain or appears after staining
speaks not only to the visible . . . sections cut from the brain for
fixation after cryopreservation? Was the fixative warm or
cold . . . minutes of warm ischemia followed by 24
hours on ice (with blood present). My *impression* (06 Jul 95 02:55:52, 19 KB) |
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# 10355 | Re: CryoNet #10344 Suspension Damage [Paul Wakfer] |
the effect that the molecules in our brain are
> broken down to smaller ones. Given . . . room temperature this requires at least 12
> hours. As you know, cryonicists attempt to get . . . loss of order,
reduction to lowest energy states, diffusion of solutes to
equiconcentration throughout, etc.) . . . experiments that such decay is fully
reversible after 17 minutues of normothermic ischemia and after (Sun, 30 Aug 1998, 8 KB) |
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# 7454 | Suicide and cryonics [Michael Darwin] |
not somebody who decided to do this after they got sick,
>society -- even I -- would . . . proposition as
has been so often been stated by its promoters (including me, in the . . . dying -- all to provide protection for their
brains in the hope of return to life . . . find a glioblastoma eating away at your brain and by the time
your quality of . . . you won't have much of a brain left either. Many
people without primary brain disease nevertheless find themselves with
metastases of cancer to their brain which present terrible choices. I watched
Jim . . . cards with friends and then a few hours later be
perfusing him.
These are, however, (08 Jan 97 07:13:28, 8 KB) |
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# 24245 | Oreg'n, Asst'd Suic'de & Hypoth'rmia [RAMole] |
terminal agonies and deterioration of
body and brain, and to die at a known time . . . promptly, and to die of hypothermia so brain deterioration is delayed for 45 minutes
or even several hours, Oregon's Assisted Suicide Law may provide . . . coldness of the water. Within a half hour the water would be cold, and within
an hour or two he'd die of hypothermia, . . . and plenty of time to work before
brain deterioration began.
Details:
Too many cryo patients die, then lie around for hours before they are
discovered to be dead, . . . they die at normal body
temperature, the brain deteriorates beyond recovery by conventional medical technology . . . everyone present, and
to die cold so brain deterioration is delayed for a long time.
. . . out and submitted. (Maybe not submitted
until after death; this part is not clear to . . . minutes. And dies in one to three hours,
usually.
The above fills all requirements except . . . actual Oregon statute at
http://www.leg.state.or.us/ors/127.html
Here is (Mon, 14 Jun 2004, 24 KB) |
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