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Msg | Description |
# 9388 | Cryopreservation of Hippocampal Slices [Paul Wakfer] |
seeks to determine optimal methods for preserving brain
slices (hippocampal slices) at ultralow temperatures. Slices . . . Alzheimer's disease, Parkinson's, and ischemic brain
damage. These methods could greatly reduce the . . . warming rates, temperature
minima, and storage times. After cryopreservation, the slices will be
rewarmed, washed . . . development of methods for
preservation of uniform brain slices from both normal and pathological
specimens . . . experimentation and thereby reduce costs. Specialized
cryopreserved brain slices could also have potential future medical
. . . as synaptic transmission and action potential propagation
after the cryopreservation and rewarming of central nervous . . . work showing successful
preservation of synaptic function after freezing and thawing the
superior cervical ganglion, . . . CNS structures important for the understanding of brain
function and pathology.
The hippocampus is an . . . formation and dissolution to be
entirely avoided. After 17 years of development, enough has been . . . followed. This method is
described below. Only after cryoprotection is optimized can the best
results . . . that
cryoprotectants exert toxicity in the frozen state, and he was the
originator of a . . . Salary: Dr. Yuri Pichugin, Research Associate, 40 hours per
week 40,000
Cost of Fringe
(Wed, 01 Apr 1998, 16 KB) |
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# 7156 | Professionalism and other topics [Charles Platt] |
Ettinger:
> Yes, in Michigan (and probably other states) the law provides that no one
> except . . . or inject a dead person--
Which other states have such a law? Does anyone have . . . and observation of the
capillaries in the brain using fiber optic scopes? No way! So-- . . . cryonics lab where perfusion will take place
after an interval of 12 to 24 hours?
What are the results suggesting that promptness (Mon, 18 Nov 1996, 6 KB) |
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# 15505 | Ongoing Cryoscience Comments - CryoNet #15482, 15483, 15485, 15489, [Paul Antonik Wakfer] |
and somewhat confused) to find Douglas Skrecky stating that the 75% solution
> of glycerol used . . . slowly,
> at less than 1 degree per hour. At Alcor, I am told that the rate is
> 10 degrees per hour, to get the temperature down as quickly . . . findings (specimens from different sites in the brain)
> do not indicate that.
This is very . . . mixed
fresh every time.
> 8. He writes:
>
> > After you wash out the blood, it would (Wed, 31 Jan 2001, 26 KB) |
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# 9299 | Re: CryoNet #9284 - #9292 [Mike Perry] |
ve
noted.
As for neurons and my state of knowledge thereof, I'll give them
. . . I think). But a neuron and a brain are
constructs made of atoms, thus in . . . sure you are not paid by the hour!) and have a lot
more time for . . . t see so much superiority here. The brain isn't
particularly good at replacing damaged parts. Moreover,
though memories are stored
redundantly in the brain, computers seem superior in providing for
"restarting" after hardware failure, since programs can be copied (Mon, 16 Mar 98, 5 KB) |
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# 5909 | Re: Virtue of suffering [John Sharman] |
dishonesty
4. Funding lost to act of State (lawful confiscation)
5. Continued cryopreservation declared illegal
. . . of liquid N2 due to social or State emergency
14. Human error in maintenance procedures
. . . m
seriously contemplating taking up smoking again after a break of many
months.
> > > What would . . . their former life restored. I am simply stating that it *may* be
> possible to recover . . . little electrical
> > activity in the thawed out brain of a frozen cat. What do you get from
> > the brain of a cat that's been lying . . . to look at it. Now you baldly state that I have
> no evidence. This is . . . time?
> The reconstruction of memories from
> cryopreserved brain tissues, while certainly beyond imagination today,
> *may* . . . can all share your
> conviction.
Sure. The brain is inhabited by fourth dimensional demons. Their
. . . on this note.
As I have clearly stated (with examples), I don't like the . . . way to put in a few extra hours to somehow get another,
> further $100,000 :-)
(Fri, 08 Mar 96, 20 KB) |
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# 11743 | False Antitheses [Davpascal] |
dies, he may face minutes if not hours before being found and
taken to a . . . are.
That ischemia causes damage to the brain is (obviously) not disputed. The
question is: . . . is, of course, a truism: if your brain is tossed at Ground
Zero when the . . . and, say, one or two or six hours of warm
ischemia are not the same . . . Of course. The alternative
is certain obliteration, after all, and a gamble in which at . . . what we were as ten-year-olds; after all, we've
forgotten things; we've changed our opinions; every molecule in our brain has
been replaced more than once, and . . . to the head, just die because their brains are not
immaculate xeroxes of what they . . . stroke in which massive areas of his brain were
destroyed. Memory lapses, paralysis, personality change - . . . a snap of the fingers a
decade after Y2K, they'll court such damage cavalierly. . . . there might not be something in religion after all: people on both sides of
the . . . Resurrection foreshadowed, as it were, the welfare state, psychotherapy, and
cryostasis. Like Goethe's Eternal (Thu, 13 May 1999, 22 KB) |
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# 6103 | Dead While Alive (again) [Brian Wowk] |
analog. Locked-in
syndrome occurs when the brain stem is destroyed by stroke
or other injury, leaving the patient's brain conscious
but incapable of sustaining life.
***************************************************************************
Brian . . . of you). I will share our experiences after
> some replies.
>
> Middle-aged patient, normally well, fronts to St Elsewheres after collapse -
> is intubated and ventilated for hypoventilation . . . would not want to remain in this
state, but she very clearly expressed her desire . . . law is very clear about this issue). After three days,
however, she decided that she . . . out. If they die
in a few hours, they stay in the unit. If they (19 Apr 96 00:21:21, 7 KB) |
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# 6776 | SCI. Fahy article [Ettinger] |
next higher concentration, 100% survival was attained
after cooling to - 32 C. These results emboldened . . . here. We are not given any clearly stated data on vitrification
temperatures (or glass transition . . . kidneys survived, with good life support function after
transplantation. Another of them also survived after further cooling to - 46
C.
Tissue slice . . . the researchers of that
time knew and stated clearly that methods required for other types . . . CRYOPRESERVATION
REQUIREMENTS BETWEEN DIFFERENT REGIONS OF THE BRAIN THAN BETWEEN (SAY) HEARTS
AND LIVERS.
Olga . . . I don't know how many
person-hours were involved or how much money, but (Sat, 17 Aug 1996, 8 KB) |
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# 16595 | Heterosexuals, cryonics and the meaning of life [Mgdarwin] |
is something
else altogether, and is a state of mind very cognizant of the problems . . . changing environment. It doesn't take
great
>
> > brains to realize that we have the wild . . . 50,000 feet, cross the world in hours, get information at
near the speed of . . . Go figure.
I am in this sorry state because we are creatures of unlimited desires . . . mechanisms like the separation of church and state, the Bill of Rights and
the like, . . . where they did reproduce it was usually after their period
of activism or after their (Tue, 19 Jun 2001, 22 KB) |
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# 12019 | Just The Facts [Davpascal] |
the processes of ischemic injury to the brain are extremely
complex and not yet fully . . . complex,
therefore everyone who drowns is irretrievably brain-dead? Well -- that's,
um, not so.
<< . . . agree with you, Charles,
for the reasons stated above, but I don't call you . . . only useful if you apply it sensibly. After you read your
piece of paper every . . . have a fresh quotation from the man stating that, "ahhh, cryonics is a lotta
bunk . . . human mind,
knows nothing about the human brain, is ludicrous. Go read some books and
. . . Smaller, 1996 Nobel Prize
Winner for Chemistry, stated that he expected them to be in . . . someone placed in cryonics suspension today
-- even after long periods of ischemia and even taken . . . Thirty years
ago, vitrification, nano-scale engineering, brain cell regeneration, cloning,
were sci-fi absurdities. . . . How are we 'risking our
credibility' by stating the fact? "Dr K. Eric Drexler, PhD, . . . PhD profiled by Time Magazine who flatly states that we have very
good reason to . . . too. Check out the CI membership form after you re-read The Society Of
Mind.
<< . . . the treatment they are likely to receive after
legal death, and the concomitant damage which . . . Ralph Merkle's Molecular Repair of the Brain essay at his Xerox
PARC website.)
Now . . . a 'conservative'
organization dies alone and undergoes hours or days of lengthy ischemia, or
dies (Sat, 26 Jun 1999, 29 KB) |
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